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dc.contributor.authorYe, Hongtao
dc.contributor.authorGong, Liping
dc.contributor.authorLiu, H
dc.contributor.authorHamoudi, Rifat A
dc.contributor.authorShirali, Sima
dc.contributor.authorHo, Liza
dc.contributor.authorChott, Andreas
dc.contributor.authorStreubel, Berthold
dc.contributor.authorSiebert, Reiner
dc.contributor.authorGesk, Stefan
dc.contributor.authorMartin-Subero, Jose I
dc.contributor.authorRadford, John A
dc.contributor.authorBanerjee, Saumitra S
dc.contributor.authorNicholson, Andrew G
dc.contributor.authorRanaldi, Renzo
dc.contributor.authorRemstein, Ellen D
dc.contributor.authorGao, Zifen
dc.contributor.authorZheng, Jie
dc.contributor.authorIsaacson, Peter G
dc.contributor.authorDogan, Ahmet
dc.contributor.authorDu, Ming-Qing
dc.date.accessioned2009-08-06T15:59:00Z
dc.date.available2009-08-06T15:59:00Z
dc.date.issued2005-02
dc.identifier.citationMALT lymphoma with t(14;18)(q32;q21)/IGH-MALT1 is characterized by strong cytoplasmic MALT1 and BCL10 expression. 2005, 205 (3):293-301 J. Pathol.en
dc.identifier.issn0022-3417
dc.identifier.pmid15682443
dc.identifier.doi10.1002/path.1715
dc.identifier.urihttp://hdl.handle.net/10541/76584
dc.description.abstractMucosa-associated lymphoid tissue (MALT) lymphoma is specifically associated with t(11;18)(q21;q21), t(1;14)(p22;q32) and t(14;18)(q32;q21). t(11;18)(q21;q21) fuses the N-terminus of the API2 gene to the C-terminus of the MALT1 gene and generates a functional API2-MALT1 product. t(1;14)(p22;q32) and t(14;18)(q32;q21) bring the BCL10 and MALT1 genes respectively to the IGH locus and deregulate their expression. The oncogenic activity of the three chromosomal translocations is linked by the physiological role of BCL10 and MALT1 in antigen receptor-mediated NFkappaB activation. In this study, MALT1 and BCL10 expression was examined in normal lymphoid tissues and 423 cases of MALT lymphoma from eight sites, and their expression was correlated with the above translocations, which were detected by molecular and molecular cytogenetic methods. In normal B-cell follicles, both MALT1 and BCL10 were expressed predominantly in the cytoplasm, high in centroblasts, moderate in centrocytes and weak/negative in mantle zone B-cells. In MALT lymphoma, MALT1 and BCL10 expression varied among cases with different chromosomal translocations. In 9/9 MALT lymphomas with t(14;18)(q32;q21), tumour cells showed strong homogeneous cytoplasmic expression of both MALT1 and BCL10. In 12/12 cases with evidence of t(1;14)(p22;q32) or variants, tumour cells expressed MALT1 weakly in the cytoplasm but BCL10 strongly in the nuclei. In all 67 MALT lymphomas with t(11;18)(q21;q21), tumour cells expressed weak cytoplasmic MALT1 and moderate nuclear BCL10. In MALT lymphomas without the above translocations, both MALT1 and BCL10, in general, were expressed weakly in the cytoplasm. Real-time quantitative RT-PCR showed a good correlation between MALT1 and BCL10 mRNA expression and underlining genetic changes, with t(14;18)(q32;q21)- and t(1;14)(p22;q32)-positive cases displaying the highest MALT1 and BCL10 mRNA expression respectively. These results show that MALT1 expression pattern is identical to that of BCL10 in normal lymphoid tissues but varies in MALT lymphomas, with high cytoplasmic expression of both MALT1 and BCL10 characterizing those with t(14;18)(q32;q21).
dc.language.isoenen
dc.subjectCancer Proteinsen
dc.subject.meshAdaptor Proteins, Signal Transducing
dc.subject.meshCaspases
dc.subject.meshChromosomes, Human, Pair 14
dc.subject.meshChromosomes, Human, Pair 18
dc.subject.meshCytoplasm
dc.subject.meshFemale
dc.subject.meshGene Expression
dc.subject.meshHumans
dc.subject.meshLymphoid Tissue
dc.subject.meshLymphoma, B-Cell, Marginal Zone
dc.subject.meshMale
dc.subject.meshNeoplasm Proteins
dc.subject.meshRNA, Messenger
dc.subject.meshRNA, Neoplasm
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
dc.subject.meshTranslocation, Genetic
dc.titleMALT lymphoma with t(14;18)(q32;q21)/IGH-MALT1 is characterized by strong cytoplasmic MALT1 and BCL10 expression.en
dc.typeArticleen
dc.contributor.departmentDepartment of Pathology, University of Cambridge, Cambridge, UK.en
dc.identifier.journalThe Journal of Pathologyen
html.description.abstractMucosa-associated lymphoid tissue (MALT) lymphoma is specifically associated with t(11;18)(q21;q21), t(1;14)(p22;q32) and t(14;18)(q32;q21). t(11;18)(q21;q21) fuses the N-terminus of the API2 gene to the C-terminus of the MALT1 gene and generates a functional API2-MALT1 product. t(1;14)(p22;q32) and t(14;18)(q32;q21) bring the BCL10 and MALT1 genes respectively to the IGH locus and deregulate their expression. The oncogenic activity of the three chromosomal translocations is linked by the physiological role of BCL10 and MALT1 in antigen receptor-mediated NFkappaB activation. In this study, MALT1 and BCL10 expression was examined in normal lymphoid tissues and 423 cases of MALT lymphoma from eight sites, and their expression was correlated with the above translocations, which were detected by molecular and molecular cytogenetic methods. In normal B-cell follicles, both MALT1 and BCL10 were expressed predominantly in the cytoplasm, high in centroblasts, moderate in centrocytes and weak/negative in mantle zone B-cells. In MALT lymphoma, MALT1 and BCL10 expression varied among cases with different chromosomal translocations. In 9/9 MALT lymphomas with t(14;18)(q32;q21), tumour cells showed strong homogeneous cytoplasmic expression of both MALT1 and BCL10. In 12/12 cases with evidence of t(1;14)(p22;q32) or variants, tumour cells expressed MALT1 weakly in the cytoplasm but BCL10 strongly in the nuclei. In all 67 MALT lymphomas with t(11;18)(q21;q21), tumour cells expressed weak cytoplasmic MALT1 and moderate nuclear BCL10. In MALT lymphomas without the above translocations, both MALT1 and BCL10, in general, were expressed weakly in the cytoplasm. Real-time quantitative RT-PCR showed a good correlation between MALT1 and BCL10 mRNA expression and underlining genetic changes, with t(14;18)(q32;q21)- and t(1;14)(p22;q32)-positive cases displaying the highest MALT1 and BCL10 mRNA expression respectively. These results show that MALT1 expression pattern is identical to that of BCL10 in normal lymphoid tissues but varies in MALT lymphomas, with high cytoplasmic expression of both MALT1 and BCL10 characterizing those with t(14;18)(q32;q21).


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