Segregation of genomes in polyploid tumour cells following mitotic catastrophe.
Authors
Erenpreisa, JekaterinaKalejs, Martins
Ianzini, Fiorenza
Kosmacek, Elizabeth A
Mackey, Mike
Emzinsh, Dzintars
Cragg, Mark S
Ivanov, Andrei
Illidge, Timothy M
Affiliation
Biomedicine Centre of the Latvia University, Ratsupites 1, Riga LV-1067, Latvia. katrina@biomed.lu.lvIssue Date
2005-12
Metadata
Show full item recordAbstract
Following irradiation p53-function-deficient tumour cells undergo mitotic catastrophe and form endopolyploid cells. A small proportion of these segregates nuclei, and give rise to viable descendants. Here we studied this process in five tumour cell lines. After mitotic failure, tumour cells enter the endocycle and form mono-nucleated or multi-nucleated giant cells (MOGC and MNGC). MNGC arise from arrested anaphases, MOGC, from arrested metaphases. In both cases the individual genomes establish a radial pattern by links to a single microtubule organizing centre. Segregation of genomes is also ordered. MNGC present features of mitosis being resumed from late anaphase. In MOGC the sub-nuclei retain arrangement of stacked metaphase plates and are separated by folds of the nuclear envelope. Mitosis then resumes in sub-nuclei directly from metaphase. The data presented indicate that endopolyploid tumour cells preserve the integrity of individual genomes and can potentially re-initiate mitosis from the point at which it was interrupted.Citation
Segregation of genomes in polyploid tumour cells following mitotic catastrophe. 2005, 29 (12):1005-11 Cell Biol. Int.Journal
Cell Biology InternationalDOI
10.1016/j.cellbi.2005.10.008PubMed ID
16314119Type
ArticleLanguage
enISSN
1065-6995ae974a485f413a2113503eed53cd6c53
10.1016/j.cellbi.2005.10.008
Scopus Count
Collections
Related articles
- Volume increase and spatial shifts of chromosome territories in nuclei of radiation-induced polyploidizing tumour cells.
- Authors: Schwarz-Finsterle J, Scherthan H, Huna A, González P, Mueller P, Schmitt E, Erenpreisa J, Hausmann M
- Issue date: 2013 Aug 30
- Mitotic catastrophe and endomitosis in tumour cells: an evolutionary key to a molecular solution.
- Authors: Erenpreisa J, Kalejs M, Cragg MS
- Issue date: 2005 Dec
- Spindle checkpoint function is required for mitotic catastrophe induced by DNA-damaging agents.
- Authors: Nitta M, Kobayashi O, Honda S, Hirota T, Kuninaka S, Marumoto T, Ushio Y, Saya H
- Issue date: 2004 Aug 26
- G1 tetraploidy checkpoint and the suppression of tumorigenesis.
- Authors: Margolis RL, Lohez OD, Andreassen PR
- Issue date: 2003 Mar 1
- Polyploid giant cells provide a survival mechanism for p53 mutant cells after DNA damage.
- Authors: Illidge TM, Cragg MS, Fringes B, Olive P, Erenpreisa JA
- Issue date: 2000