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dc.contributor.authorBarrow, Katie M
dc.contributor.authorWard, Christopher M
dc.contributor.authorRutter, Jennifer
dc.contributor.authorAli, Sumia
dc.contributor.authorStern, Peter L
dc.date.accessioned2009-07-24T15:39:29Z
dc.date.available2009-07-24T15:39:29Z
dc.date.issued2005-08
dc.identifier.citationEmbryonic expression of murine 5T4 oncofoetal antigen is associated with morphogenetic events at implantation and in developing epithelia. 2005, 233 (4):1535-45 Dev. Dyn.en
dc.identifier.issn1058-8388
dc.identifier.pmid15977177
dc.identifier.doi10.1002/dvdy.20482
dc.identifier.urihttp://hdl.handle.net/10541/75664
dc.description.abstractOverexpression of 5T4 oncofoetal antigen, an early marker of ES cell differentiation, in vitro increases cellular motility and decreases adhesion, properties relevant to development and cancer. Embryonic expression of m5T4 antigen is first detected on trophectoderm at implantation and is restricted to extra-embryonic tissues to embryonic day (E) 11.5. In the embryo, significant m5T4 expression is detected at E12.5 in hindbrain roofplate and in various epithelia derived from all germ layers. In keratin 14-expressing epithelia, there is a congruent 5T4 expression pattern with many of these cells being Ki-67 positive. In brain, expression is observed in roofplate, ependymal layers, choroid plexus, and subventricular zones of lateral ventricles at E14.5. By E17.5, expression is decreased in the subventricular zone with further restriction to choroid plexus in adult brain. Our data demonstrate a limited 5T4 expression profile during embryogenesis associated with actively cycling, undifferentiated epithelial progenitor cells that may contribute to their migration.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshAntigens, Surface
dc.subject.meshBlastocyst
dc.subject.meshCell Line
dc.subject.meshCell Movement
dc.subject.meshEmbryo Implantation
dc.subject.meshEmbryonic Development
dc.subject.meshEpithelium
dc.subject.meshFluorescent Antibody Technique, Indirect
dc.subject.meshKeratinocytes
dc.subject.meshMembrane Glycoproteins
dc.subject.meshMice
dc.subject.meshNervous System
dc.subject.meshPregnancy Proteins
dc.subject.meshZona Pellucida
dc.titleEmbryonic expression of murine 5T4 oncofoetal antigen is associated with morphogenetic events at implantation and in developing epithelia.en
dc.typeArticleen
dc.contributor.departmentCR UK Immunology Group, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, United Kingdom.en
dc.identifier.journalDevelopmental Dynamicsen
html.description.abstractOverexpression of 5T4 oncofoetal antigen, an early marker of ES cell differentiation, in vitro increases cellular motility and decreases adhesion, properties relevant to development and cancer. Embryonic expression of m5T4 antigen is first detected on trophectoderm at implantation and is restricted to extra-embryonic tissues to embryonic day (E) 11.5. In the embryo, significant m5T4 expression is detected at E12.5 in hindbrain roofplate and in various epithelia derived from all germ layers. In keratin 14-expressing epithelia, there is a congruent 5T4 expression pattern with many of these cells being Ki-67 positive. In brain, expression is observed in roofplate, ependymal layers, choroid plexus, and subventricular zones of lateral ventricles at E14.5. By E17.5, expression is decreased in the subventricular zone with further restriction to choroid plexus in adult brain. Our data demonstrate a limited 5T4 expression profile during embryogenesis associated with actively cycling, undifferentiated epithelial progenitor cells that may contribute to their migration.


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