Embryonic expression of murine 5T4 oncofoetal antigen is associated with morphogenetic events at implantation and in developing epithelia.
AffiliationCR UK Immunology Group, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, United Kingdom.
MetadataShow full item record
AbstractOverexpression of 5T4 oncofoetal antigen, an early marker of ES cell differentiation, in vitro increases cellular motility and decreases adhesion, properties relevant to development and cancer. Embryonic expression of m5T4 antigen is first detected on trophectoderm at implantation and is restricted to extra-embryonic tissues to embryonic day (E) 11.5. In the embryo, significant m5T4 expression is detected at E12.5 in hindbrain roofplate and in various epithelia derived from all germ layers. In keratin 14-expressing epithelia, there is a congruent 5T4 expression pattern with many of these cells being Ki-67 positive. In brain, expression is observed in roofplate, ependymal layers, choroid plexus, and subventricular zones of lateral ventricles at E14.5. By E17.5, expression is decreased in the subventricular zone with further restriction to choroid plexus in adult brain. Our data demonstrate a limited 5T4 expression profile during embryogenesis associated with actively cycling, undifferentiated epithelial progenitor cells that may contribute to their migration.
CitationEmbryonic expression of murine 5T4 oncofoetal antigen is associated with morphogenetic events at implantation and in developing epithelia. 2005, 233 (4):1535-45 Dev. Dyn.
- The 5T4 oncofoetal antigen is an early differentiation marker of mouse ES cells and its absence is a useful means to assess pluripotency.
- Authors: Ward CM, Barrow K, Woods AM, Stern PL
- Issue date: 2003 Nov 15
- Metastasis-associated 5T4 oncofoetal antigen is concentrated at microvillus projections of the plasma membrane.
- Authors: Carsberg CJ, Myers KA, Evans GS, Allen TD, Stern PL
- Issue date: 1995 Aug
- Cell surface 5T4 antigen is transiently upregulated during early human embryonic stem cell differentiation: effect of 5T4 phenotype on neural lineage formation.
- Authors: Ward CM, Eastham AM, Stern PL
- Issue date: 2006 Jun 10
- Differential expression of ezrin/radixin/moesin (ERM) and ERM-associated adhesion molecules in the blastocyst and uterus suggests their functions during implantation.
- Authors: Matsumoto H, Daikoku T, Wang H, Sato E, Dey SK
- Issue date: 2004 Mar
- Implantation mechanisms: insights from the sheep.
- Authors: Spencer TE, Johnson GA, Bazer FW, Burghardt RC
- Issue date: 2004 Dec