• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Opticin binds to heparan and chondroitin sulfate proteoglycans.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Hindson, V John
    Gallagher, John T
    Halfter, Willi
    Bishop, Paul N
    Affiliation
    Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences and Academic Unit of Eye & Vision Science, School of Medicine, University of Manchester, United Kingdom.
    Issue Date
    2005-12
    
    Metadata
    Show full item record
    Abstract
    PURPOSE: The extracellular matrix glycoprotein opticin is a small leucine-rich repeat proteoglycan/protein family member that was discovered associated with vitreous humor collagen fibrils. Opticin is present throughout the vitreous, but is particularly concentrated at the internal limiting lamina, where it colocalizes with type XVIII collagen. The present study investigated whether opticin interacts directly with the heparan sulfate (HS) proteoglycan type XVIII collagen. METHODS: Solid-phase opticin binding assays were performed with immobilized type XVIII collagen and heparin albumin. Surface plasmon resonance (SPR) was used to investigate the binding of opticin to heparin and HS. RESULTS: Opticin bound to type XVIII collagen via its HS chains. SPR showed that opticin bound to porcine intestinal mucosa HS and heparin with moderately high affinity (K(D) 73 and 43 nM, respectively). Binding inhibition studies showed that hexasaccharides of heparin had a lower affinity for opticin than larger oligosaccharides; the sulfate groups of heparin contributed variably to opticin binding, with the group at ring position two of iduronate contributing least; and chondroitin sulfate A and B bound to opticin, whereas binding to chondroitin sulfate C and hyaluronan was not observed. CONCLUSIONS: Opticin binds to heparin, HS, chondroitin 4-sulfate, and dermatan sulfate, the binding affinity being dependent on sulfation pattern and oligosaccharide chain length. Opticin may provide a link between cortical vitreous collagen fibrils and the inner limiting lamina by binding HS proteoglycans and stabilize vitreous gel structure by binding chondroitin sulfate proteoglycans.
    Citation
    Opticin binds to heparan and chondroitin sulfate proteoglycans. 2005, 46 (12):4417-23 Invest. Ophthalmol. Vis. Sci.
    Journal
    Investigative Ophthalmology & Visual Science
    URI
    http://hdl.handle.net/10541/75656
    DOI
    10.1167/iovs.05-0883
    PubMed ID
    16303928
    Type
    Article
    Language
    en
    ISSN
    0146-0404
    ae974a485f413a2113503eed53cd6c53
    10.1167/iovs.05-0883
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • Perlecan is responsible for thrombospondin 1 binding on the cell surface of cultured porcine endothelial cells.
    • Authors: Vischer P, Feitsma K, Schön P, Völker W
    • Issue date: 1997 Aug
    • Differential binding of vascular cell-derived proteoglycans (perlecan, biglycan, decorin, and versican) to the beta-amyloid protein of Alzheimer's disease.
    • Authors: Snow AD, Kinsella MG, Parks E, Sekiguchi RT, Miller JD, Kimata K, Wight TN
    • Issue date: 1995 Jun 20
    • Chondroitin sulfate proteoglycans are structural renewable constituents of the rabbit vitreous body.
    • Authors: Góes RM, Nader HB, Porcionatto MA, Haddad A, Laicine EM
    • Issue date: 2005 May
    • Hyaluronan and chondroitin sulfate proteoglycans in the supramolecular organization of the mammalian vitreous body.
    • Authors: Theocharis DA, Skandalis SS, Noulas AV, Papageorgakopoulou N, Theocharis AD, Karamanos NK
    • Issue date: 2008
    • Secreted chondroitin sulfate proteoglycan of human B cell lines binds to the complement protein C1q and inhibits complex formation of C1.
    • Authors: Kirschfink M, Blase L, Engelmann S, Schwartz-Albiez R
    • Issue date: 1997 Feb 1
    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.