Estimation of haemopoietic progenitor cells in peripheral blood by the Advia 120 and BD vantage flow cytometer: a direct comparison for the prediction of adequate collections.
Authors
Greenfield, Hayley MSweeney, D A
Newton, R K
Leather, Angela
Murray, John
Angelica, R
Swindell, Ric
Chang, James
Affiliation
Department of Haematology, Christie Hospital, Manchester, UK. hayleygreenfield@tiscali.co.ukIssue Date
2005-10
Metadata
Show full item recordAbstract
Peripheral blood stem cells are increasingly used to ensure rapid haematological engraftment after myeloablative chemotherapy. After mobilization, progenitor cells in the blood can be enumerated to predict an adequate collection by leukapheresis. The Advia 120 automated counter has an immature cell channel measuring a parameter known as large undifferentiated cells (LUC's), which were quantified to assess their value in refining the timing of apheresis. Data were available from 102 apheresis sessions. Positive correlation was found for peripheral blood CD34+ cells and apheresis counts (r = 0.82, P < 0.0005) but not for total WCC (r = -0.15, P = 0.13) or LUC count (r = 0.12, P = 0.23). Our results indicate that the LUC population in peripheral blood has no relevance to the subsequent CD34 content of the apheresis product and CD34 cell enumeration by flow cytometry is advocated.Citation
Estimation of haemopoietic progenitor cells in peripheral blood by the Advia 120 and BD vantage flow cytometer: a direct comparison for the prediction of adequate collections. 2005, 27 (5):287-91 Clin Lab HaematolJournal
Clinical and Laboratory HaematologyDOI
10.1111/j.1365-2257.2005.00712.xPubMed ID
16178906Type
ArticleLanguage
enISSN
0141-9854ae974a485f413a2113503eed53cd6c53
10.1111/j.1365-2257.2005.00712.x