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    Multiple reaction monitoring to identify sites of protein phosphorylation with high sensitivity.

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    Authors
    Unwin, Richard D
    Griffiths, John R
    Leverentz, Michael K
    Grallert, Agnes
    Hagan, Iain M
    Whetton, Anthony D
    Affiliation
    Faculty of Medical and Human Sciences, University of Manchester, United Kingdom.
    Issue Date
    2005-08
    
    Metadata
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    Abstract
    Phosphorylation governs the activity of many proteins. Insight into molecular mechanisms in biology would be immensely improved by robust, sensitive methods for identifying precisely sites of phosphate addition. An approach to selective mapping of protein phosphorylation sites on a specific target protein of interest using LC-MS is described here. In this approach multiple reaction monitoring is used as an extremely sensitive MS survey scan for potential phosphopeptides from a known protein. This is automatically followed by peptide sequencing and subsequent location of the phosphorylation site; both of these steps occur in a single LC-MS run, providing greater efficiency of sample use. The method is capable of detecting and sequencing phosphopeptides at low femtomole levels with high selectivity. As proof of the value of this approach in an experimental setting, a key Schizosaccharomyces pombe cell cycle regulatory protein, Cyclin B, was purified, and associated proteins were identified. Phosphorylation sites on these proteins were located. The technique, which we have called multiple reaction monitoring-initiated detection and sequencing (MIDAS), is shown to be a highly sensitive approach to the determination of protein phosphorylation.
    Citation
    Multiple reaction monitoring to identify sites of protein phosphorylation with high sensitivity. 2005, 4 (8):1134-44 Mol. Cell Proteomics
    Journal
    Molecular & Cellular Proteomics
    URI
    http://hdl.handle.net/10541/74817
    DOI
    10.1074/mcp.M500113-MCP200
    PubMed ID
    15923565
    Type
    Article
    Language
    en
    ISSN
    1535-9476
    ae974a485f413a2113503eed53cd6c53
    10.1074/mcp.M500113-MCP200
    Scopus Count
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    All Paterson Institute for Cancer Research

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