Spectral discrimination of live prostate and bladder cancer cell lines using Raman optical tweezers.
Authors
Harvey, Tim JFaria, Elsa Correia
Henderson, Alex
Gazi, Ehsan
Ward, Andrew D
Clarke, Noel W
Brown, Michael D
Snook, Richard D
Gardner, Peter
Affiliation
University of Manchester, School of Chemical Engineering and Analytical Science, Manchester Interdisciplinary Biocentre, 131 Princess Street, Manchester, Manchester M1 7DN, United Kingdom.Issue Date
2009-07-16T09:11:35Z
Metadata
Show full item recordAbstract
An investigation into the use of Raman optical tweezers to study urological cell lines is reported, with the ultimate aim of determining the presence of malignant CaP cells in urine and peripheral fluids. To this end, we trapped and analyzed live CaP cells (PC-3) and bladder cells (MGH-U1), because both prostate and bladder cells are likely to be present in urine. The laser excitation wavelength of 514.5 nm was used, with Raman light collected both in back- and forward-scattering geometric configurations. For the backscattering configuration the same laser was used for trapping and excitation, while for forward scattering a 1064 nm laser provided the trapping beam. Analysis of cell-diameter distributions for cells analyzed suggested normal distribution of cell sizes, indicating an unbiased cell-selection criterion. Principal components analysis afforded discrimination of MGH-U1 and PC-3 spectra collected in either configuration, demonstrating that it is possible to trap, analyze, and differentiate PC-3 from MGH-U1 cells using a 514.5 nm laser. By loading plot analysis, possible biomolecules responsible for discrimination in both configurations were determined. Finally, the effect of cell size on discrimination was investigated, with results indicating that separation is based predominantly on cell type rather than cell size.Citation
Spectral discrimination of live prostate and bladder cancer cell lines using Raman optical tweezers., 13 (6):064004 J Biomed OptJournal
Journal of Biomedical OpticsDOI
10.1117/1.2999609PubMed ID
19123651Type
ArticleLanguage
enISSN
1083-3668ae974a485f413a2113503eed53cd6c53
10.1117/1.2999609