• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsProfilesView

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    The haemangioblast generates haematopoietic cells through a haemogenic endothelium stage.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Lancrin, Christophe
    Sroczynska, Patrycja
    Stephenson, Catherine
    Allen, Terence D
    Kouskoff, Valerie
    Lacaud, Georges
    Affiliation
    Cancer Research UK Stem Cell Biology Group.
    Issue Date
    2009-02-12
    
    Metadata
    Show full item record
    Abstract
    It has been proposed that during embryonic development haematopoietic cells arise from a mesodermal progenitor with both endothelial and haematopoietic potential called the haemangioblast. A conflicting theory instead associates the first haematopoietic cells with a phenotypically differentiated endothelial cell that has haematopoietic potential (that is, a haemogenic endothelium). Support for the haemangioblast concept was initially provided by the identification during mouse embryonic stem cell differentiation of a clonal precursor, the blast colony-forming cell (BL-CFC), which gives rise to blast colonies with both endothelial and haematopoietic components. Although recent studies have now provided evidence for the presence of this bipotential precursor in vivo, the precise mechanism for generation of haematopoietic cells from the haemangioblast still remains completely unknown. Here we demonstrate that the haemangioblast generates haematopoietic cells through the formation of a haemogenic endothelium intermediate, providing the first direct link between these two precursor populations. The cell population containing the haemogenic endothelium is transiently generated during BL-CFC development. This cell population is also present in gastrulating mouse embryos and generates haematopoietic cells on further culture. At the molecular level, we demonstrate that the transcription factor Tal1 (also known as Scl; ref. 10) is indispensable for the establishment of this haemogenic endothelium population whereas the core binding factor Runx1 (also known as AML1; ref. 11) is critical for generation of definitive haematopoietic cells from haemogenic endothelium. Together our results merge the two a priori conflicting theories on the origin of haematopoietic development into a single linear developmental process.
    Citation
    The haemangioblast generates haematopoietic cells through a haemogenic endothelium stage. 2009, 457 (7231):892-5 Nature
    Journal
    Nature
    URI
    http://hdl.handle.net/10541/74015
    DOI
    10.1038/nature07679
    PubMed ID
    19182774
    Type
    Article
    Language
    en
    ISSN
    1476-4687
    ae974a485f413a2113503eed53cd6c53
    10.1038/nature07679
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research
    Stem Cell and Haematopoiesis
    Stem Cell Biology

    entitlement

    Related articles

    • SOX7 regulates the expression of VE-cadherin in the haemogenic endothelium at the onset of haematopoietic development.
    • Authors: Costa G, Mazan A, Gandillet A, Pearson S, Lacaud G, Kouskoff V
    • Issue date: 2012 May
    • Continuous single-cell imaging of blood generation from haemogenic endothelium.
    • Authors: Eilken HM, Nishikawa S, Schroeder T
    • Issue date: 2009 Feb 12
    • Haemangioblast commitment is initiated in the primitive streak of the mouse embryo.
    • Authors: Huber TL, Kouskoff V, Fehling HJ, Palis J, Keller G
    • Issue date: 2004 Dec 2
    • HoxA3 is an apical regulator of haemogenic endothelium.
    • Authors: Iacovino M, Chong D, Szatmari I, Hartweck L, Rux D, Caprioli A, Cleaver O, Kyba M
    • Issue date: 2011 Jan
    • Early dynamic fate changes in haemogenic endothelium characterized at the single-cell level.
    • Authors: Swiers G, Baumann C, O'Rourke J, Giannoulatou E, Taylor S, Joshi A, Moignard V, Pina C, Bee T, Kokkaliaris KD, Yoshimoto M, Yoder MC, Frampton J, Schroeder T, Enver T, Göttgens B, de Bruijn MFTR
    • Issue date: 2013
    DSpace software (copyright © 2002 - 2025)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.