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    Classification of fixed urological cells using Raman tweezers.

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    Authors
    Harvey, Tim J
    Hughes, Caryn
    Ward, Andrew D
    Faria, Elsa Correia
    Henderson, Alex
    Clarke, Noel W
    Brown, Michael D
    Snook, Richard D
    Gardner, Peter
    Affiliation
    School of Chemical Engineering and Analytical Science, Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester, UK.
    Issue Date
    2009-02
    
    Metadata
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    Abstract
    In this paper we report on preliminary investigations into using Raman tweezers to classify urological cell lines. This builds on earlier work within the group, whereby Raman tweezer methodologies were developed, and the application of this technique to differentiate between live prostate cancer (CaP) and bladder cells lines (PC-3 and MGH-U1 respectively) was demonstrated.In this present study we analysed chemically fixed cells using two different fixative methods; SurePath (a commercial available liquid based cytology media) and 4% v/v formalin/PBS fixatives. The study has been expanded from our previous live cell study to include the androgen sensitive CaP cell line LNCaP, primary benign prostate hyperplasia (BPH) cells as well as primary urethral cells. Raman light from the cells was collected using a 514.5 nm Ar-ion laser excitation source in back-scattering configuration mode.Principal component-linear discriminate analysis (PC-LDA) models of resulting cell spectra were generated and these were validated using a blind comparison. Sensitivities and specificities of > 72% and 90% respectively, for SurePath fixed cells, and > 93% and 98% respectively for 4% v/v formalin/PBS fixed cells was achieved. The higher prediction results for the formalin fixed cells can be attributed to a better signal-to-noise ratio for spectra obtained from these cells.Following on from this work, urological cell lines were exposed to urine for up to 12 hours to determine the effect of urine on the ability to classify these cells. Results indicate that urine has no detrimental effect on prediction results.
    Citation
    Classification of fixed urological cells using Raman tweezers. 2009, 2 (1-2):47-69 J Biophotonics
    Journal
    Journal of Biophotonics
    URI
    http://hdl.handle.net/10541/73956
    DOI
    10.1002/jbio.200810061
    PubMed ID
    19343685
    Type
    Article
    Language
    en
    ISSN
    1864-0648
    ae974a485f413a2113503eed53cd6c53
    10.1002/jbio.200810061
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research
    School of Cancer and Imaging Sciences

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