Authors
Harvey, Tim JHughes, Caryn
Ward, Andrew D
Faria, Elsa Correia
Henderson, Alex
Clarke, Noel W
Brown, Michael D
Snook, Richard D
Gardner, Peter
Affiliation
School of Chemical Engineering and Analytical Science, Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester, UK.Issue Date
2009-02
Metadata
Show full item recordAbstract
In this paper we report on preliminary investigations into using Raman tweezers to classify urological cell lines. This builds on earlier work within the group, whereby Raman tweezer methodologies were developed, and the application of this technique to differentiate between live prostate cancer (CaP) and bladder cells lines (PC-3 and MGH-U1 respectively) was demonstrated.In this present study we analysed chemically fixed cells using two different fixative methods; SurePath (a commercial available liquid based cytology media) and 4% v/v formalin/PBS fixatives. The study has been expanded from our previous live cell study to include the androgen sensitive CaP cell line LNCaP, primary benign prostate hyperplasia (BPH) cells as well as primary urethral cells. Raman light from the cells was collected using a 514.5 nm Ar-ion laser excitation source in back-scattering configuration mode.Principal component-linear discriminate analysis (PC-LDA) models of resulting cell spectra were generated and these were validated using a blind comparison. Sensitivities and specificities of > 72% and 90% respectively, for SurePath fixed cells, and > 93% and 98% respectively for 4% v/v formalin/PBS fixed cells was achieved. The higher prediction results for the formalin fixed cells can be attributed to a better signal-to-noise ratio for spectra obtained from these cells.Following on from this work, urological cell lines were exposed to urine for up to 12 hours to determine the effect of urine on the ability to classify these cells. Results indicate that urine has no detrimental effect on prediction results.Citation
Classification of fixed urological cells using Raman tweezers. 2009, 2 (1-2):47-69 J BiophotonicsJournal
Journal of BiophotonicsDOI
10.1002/jbio.200810061PubMed ID
19343685Type
ArticleLanguage
enISSN
1864-0648ae974a485f413a2113503eed53cd6c53
10.1002/jbio.200810061
Scopus Count
Related articles
- Spectral discrimination of live prostate and bladder cancer cell lines using Raman optical tweezers.
- Authors: Harvey TJ, Faria EC, Henderson A, Gazi E, Ward AD, Clarke NW, Brown MD, Snook RD, Gardner P
- Issue date: 2008 Nov-Dec
- Classification of formalin-fixed bladder cancer cells with laser tweezer Raman spectroscopy.
- Authors: Tang NT, Robinson R, Snook RD, Brown M, Clarke N, Gardner P
- Issue date: 2023 Aug 21
- Modulated Raman spectroscopy for enhanced identification of bladder tumor cells in urine samples.
- Authors: Canetta E, Mazilu M, De Luca AC, Carruthers AE, Dholakia K, Neilson S, Sargeant H, Briscoe T, Herrington CS, Riches AC
- Issue date: 2011 Mar
- The use of Raman spectroscopy to differentiate between different prostatic adenocarcinoma cell lines.
- Authors: Crow P, Barrass B, Kendall C, Hart-Prieto M, Wright M, Persad R, Stone N
- Issue date: 2005 Jun 20
- Raman spectroscopy of bladder tissue in the presence of 5-aminolevulinic acid.
- Authors: Grimbergen MC, van Swol CF, van Moorselaar RJ, Uff J, Mahadevan-Jansen A, Stone N
- Issue date: 2009 Jun 3