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dc.contributor.authorAraki, Yasuhiro
dc.contributor.authorLau, Corine K
dc.contributor.authorMaekawa, Hiromi
dc.contributor.authorJaspersen, Sue L
dc.contributor.authorGiddings, Thomas H
dc.contributor.authorSchiebel, Elmar
dc.contributor.authorWiney, Mark
dc.date.accessioned2009-07-09T12:22:50Z
dc.date.available2009-07-09T12:22:50Z
dc.date.issued2006-04
dc.identifier.citationThe Saccharomyces cerevisiae spindle pole body (SPB) component Nbp1p is required for SPB membrane insertion and interacts with the integral membrane proteins Ndc1p and Mps2p. 2006, 17 (4):1959-70 Mol. Biol. Cellen
dc.identifier.issn1059-1524
dc.identifier.pmid16436507
dc.identifier.doi10.1091/mbc.E05-07-0668
dc.identifier.urihttp://hdl.handle.net/10541/73117
dc.description.abstractThe spindle pole body (SPB) in Saccharomyces cerevisiae functions to nucleate and organize spindle microtubules, and it is embedded in the nuclear envelope throughout the yeast life cycle. However, the mechanism of membrane insertion of the SPB has not been elucidated. Ndc1p is an integral membrane protein that localizes to SPBs, and it is required for insertion of the SPB into the nuclear envelope during SPB duplication. To better understand the function of Ndc1p, we performed a dosage suppressor screen using the ndc1-39 temperature-sensitive allele. We identified an essential SPB component, Nbp1p. NBP1 shows genetic interactions with several SPB genes in addition to NDC1, and two-hybrid analysis revealed that Nbp1p binds to Ndc1p. Furthermore, Nbp1p is in the Mps2p-Bbp1p complex in the SPB. Immunoelectron microscopy confirmed that Nbp1p localizes to the SPB, suggesting a function at this location. Consistent with this hypothesis, nbp1-td (a degron allele) cells fail in SPB duplication upon depletion of Nbp1p. Importantly, these cells exhibit a "dead" SPB phenotype, similar to cells mutant in MPS2, NDC1, or BBP1. These results demonstrate that Nbp1p is a SPB component that acts in SPB duplication at the point of SPB insertion into the nuclear envelope.
dc.language.isoenen
dc.subject.meshAlleles
dc.subject.meshCarrier Proteins
dc.subject.meshCell Cycle
dc.subject.meshCell Cycle Proteins
dc.subject.meshCell Membrane
dc.subject.meshCytoskeletal Proteins
dc.subject.meshDNA-Binding Proteins
dc.subject.meshGenes, Essential
dc.subject.meshGenes, Fungal
dc.subject.meshMembrane Proteins
dc.subject.meshMicrotubule Proteins
dc.subject.meshMicrotubules
dc.subject.meshMitosis
dc.subject.meshMitotic Spindle Apparatus
dc.subject.meshNuclear Envelope
dc.subject.meshNuclear Proteins
dc.subject.meshSaccharomyces cerevisiae
dc.subject.meshSaccharomyces cerevisiae Proteins
dc.subject.meshTwo-Hybrid System Techniques
dc.titleThe Saccharomyces cerevisiae spindle pole body (SPB) component Nbp1p is required for SPB membrane insertion and interacts with the integral membrane proteins Ndc1p and Mps2p.en
dc.typeArticleen
dc.contributor.departmentThe Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester M20 4BX, United Kingdom.en
dc.identifier.journalMolecular Biology of the Cellen
html.description.abstractThe spindle pole body (SPB) in Saccharomyces cerevisiae functions to nucleate and organize spindle microtubules, and it is embedded in the nuclear envelope throughout the yeast life cycle. However, the mechanism of membrane insertion of the SPB has not been elucidated. Ndc1p is an integral membrane protein that localizes to SPBs, and it is required for insertion of the SPB into the nuclear envelope during SPB duplication. To better understand the function of Ndc1p, we performed a dosage suppressor screen using the ndc1-39 temperature-sensitive allele. We identified an essential SPB component, Nbp1p. NBP1 shows genetic interactions with several SPB genes in addition to NDC1, and two-hybrid analysis revealed that Nbp1p binds to Ndc1p. Furthermore, Nbp1p is in the Mps2p-Bbp1p complex in the SPB. Immunoelectron microscopy confirmed that Nbp1p localizes to the SPB, suggesting a function at this location. Consistent with this hypothesis, nbp1-td (a degron allele) cells fail in SPB duplication upon depletion of Nbp1p. Importantly, these cells exhibit a "dead" SPB phenotype, similar to cells mutant in MPS2, NDC1, or BBP1. These results demonstrate that Nbp1p is a SPB component that acts in SPB duplication at the point of SPB insertion into the nuclear envelope.


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