The Saccharomyces cerevisiae spindle pole body (SPB) component Nbp1p is required for SPB membrane insertion and interacts with the integral membrane proteins Ndc1p and Mps2p.
Lau, Corine K
Jaspersen, Sue L
Giddings, Thomas H
AffiliationThe Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester M20 4BX, United Kingdom.
MetadataShow full item record
AbstractThe spindle pole body (SPB) in Saccharomyces cerevisiae functions to nucleate and organize spindle microtubules, and it is embedded in the nuclear envelope throughout the yeast life cycle. However, the mechanism of membrane insertion of the SPB has not been elucidated. Ndc1p is an integral membrane protein that localizes to SPBs, and it is required for insertion of the SPB into the nuclear envelope during SPB duplication. To better understand the function of Ndc1p, we performed a dosage suppressor screen using the ndc1-39 temperature-sensitive allele. We identified an essential SPB component, Nbp1p. NBP1 shows genetic interactions with several SPB genes in addition to NDC1, and two-hybrid analysis revealed that Nbp1p binds to Ndc1p. Furthermore, Nbp1p is in the Mps2p-Bbp1p complex in the SPB. Immunoelectron microscopy confirmed that Nbp1p localizes to the SPB, suggesting a function at this location. Consistent with this hypothesis, nbp1-td (a degron allele) cells fail in SPB duplication upon depletion of Nbp1p. Importantly, these cells exhibit a "dead" SPB phenotype, similar to cells mutant in MPS2, NDC1, or BBP1. These results demonstrate that Nbp1p is a SPB component that acts in SPB duplication at the point of SPB insertion into the nuclear envelope.
CitationThe Saccharomyces cerevisiae spindle pole body (SPB) component Nbp1p is required for SPB membrane insertion and interacts with the integral membrane proteins Ndc1p and Mps2p. 2006, 17 (4):1959-70 Mol. Biol. Cell
JournalMolecular Biology of the Cell
- A novel allele of Saccharomyces cerevisiae NDC1 reveals a potential role for the spindle pole body component Ndc1p in nuclear pore assembly.
- Authors: Lau CK, Giddings TH Jr, Winey M
- Issue date: 2004 Apr
- The Bbp1p-Mps2p complex connects the SPB to the nuclear envelope and is essential for SPB duplication.
- Authors: Schramm C, Elliott S, Shevchenko A, Schiebel E
- Issue date: 2000 Feb 1
- Saccharomyces cerevisiae MPS2 encodes a membrane protein localized at the spindle pole body and the nuclear envelope.
- Authors: Muñoz-Centeno MC, McBratney S, Monterrosa A, Byers B, Mann C, Winey M
- Issue date: 1999 Jul
- A ternary membrane protein complex anchors the spindle pole body in the nuclear envelope in budding yeast.
- Authors: Kupke T, Malsam J, Schiebel E
- Issue date: 2017 May 19
- Integrity and function of the Saccharomyces cerevisiae spindle pole body depends on connections between the membrane proteins Ndc1, Rtn1, and Yop1.
- Authors: Casey AK, Dawson TR, Chen J, Friederichs JM, Jaspersen SL, Wente SR
- Issue date: 2012 Oct