AffiliationCancer Research-UK/University of Manchester Radiochemical Targeting and Imaging Group, Paterson Institute for Cancer Research, Manchester M20 4BX, UK. email@example.com
MetadataShow full item record
AbstractThis paper describes the first application of a microfabricated reaction system to positron emission tomography (PET) radiochemistry. We have applied microfluidic technology to synthesise PET radiopharmaceuticals using (18)F and (124)I as labels for fluorodeoxyglucose (FDG) and Annexin-V, respectively. These reactions involved established methods of nucleophilic substitution on a mannose triflate precursor and direct iodination of the protein using iodogen as an oxidant. This has demonstrated a proof of principle of using microfluidic technology to radiochemical reactions involving low and high molecular weight compounds. Using microfluidic reactions, [(18)F]FDG was synthesised with a 50% incorporation of the available F-18 radioactivity in a very short time of 4s. The radiolabelling efficiency of (124)I Annexin-V was 40% after 1 min reaction time. Chromatographic analysis showed that such reaction yields are comparable to conventional methods, but in a much shorter time. The yields can be further improved with more optimisation of the microfluidic device itself and its fluid mixing profiles. This demonstrates the potential for this technology to have an impact on rapid and simpler radiopharmaceutical synthesis using short and medium half-life radionuclides.
CitationMicrofluidic technology for PET radiochemistry. 2006, 64 (3):333-6 Appl Radiat Isot
JournalApplied Radiation and Isotopes
- Microfluidic reactor for the radiosynthesis of PET radiotracers.
- Authors: Gillies JM, Prenant C, Chimon GN, Smethurst GJ, Perrie W, Hamblett I, Dekker B, Zweit J
- Issue date: 2006 Mar
- Hardware and software modifications on the Advion NanoTek microfluidic platform to extend flexibility for radiochemical synthesis.
- Authors: Pascali G, Berton A, DeSimone M, Wyatt N, Matesic L, Greguric I, Salvadori PA
- Issue date: 2014 Feb
- Application of [18F]FDG in radiolabeling reactions using microfluidic technology.
- Authors: Bouvet VR, Wuest F
- Issue date: 2013 Nov 21
- Electrochemical concentration of no-carrier-added [(18)F]fluoride from [(18)O]water in a disposable microfluidic cell for radiosynthesis of (18)F-labeled radiopharmaceuticals.
- Authors: Saiki H, Iwata R, Nakanishi H, Wong R, Ishikawa Y, Furumoto S, Yamahara R, Sakamoto K, Ozeki E
- Issue date: 2010 Sep
- Radiochemistry on chip: towards dose-on-demand synthesis of PET radiopharmaceuticals.
- Authors: Arima V, Pascali G, Lade O, Kretschmer HR, Bernsdorf I, Hammond V, Watts P, De Leonardis F, Tarn MD, Pamme N, Cvetkovic BZ, Dittrich PS, Vasovic N, Duane R, Jaksic A, Zacheo A, Zizzari A, Marra L, Perrone E, Salvadori PA, Rinaldi R
- Issue date: 2013 Jun 21