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dc.contributor.authorHarrison, Kathryn L
dc.contributor.authorCrosbie, Philip A J
dc.contributor.authorAgius, Raymond M
dc.contributor.authorBarber, Philip V
dc.contributor.authorCarus, Mark
dc.contributor.authorMargison, Geoffrey P
dc.contributor.authorPovey, Andrew C
dc.date.accessioned2009-07-09T12:14:40Z
dc.date.available2009-07-09T12:14:40Z
dc.date.issued2006-08-30
dc.identifier.citationNo association between N7-methyldeoxyguanosine and 8-oxodeoxyguanosine levels in human lymphocyte DNA. 2006, 600 (1-2):125-30 Mutat. Res.en
dc.identifier.issn0027-5107
dc.identifier.pmid16765387
dc.identifier.doi10.1016/j.mrfmmm.2006.03.005
dc.identifier.urihttp://hdl.handle.net/10541/73114
dc.description.abstractTo examine associations between two different classes of DNA damage that can occur through endogenous processes or exogenous exposures such as smoking, N7-methyldeoxyguanosine (N7-MedG) and 8-oxodeoxyguanosine (8-oxodG) levels were measured in lymphocyte DNA from 22 bronchoscopy patients. 8-OxodG and N7-MedG was detected in 100% and 91% of samples, respectively with 8-oxodG levels being approx 20 times higher (mean 8.39+/-3.578-oxodG/10(6)dG versus 0.41+/-0.33 N7-MedG/10(6) dG) which provides an indication of the relative importance of the agents that induce oxidative DNA damage or alkylation damage. The sources of these genotoxic lesions remain to be established but N7-MedG and 8-oxodG levels were not correlated (r(2)<0.01) suggesting that there is no association between alkylating agent and reactive oxygen species exposure, their metabolism and/or the DNA repair processes that can remove this DNA damage.
dc.language.isoenen
dc.subject.meshAged
dc.subject.meshAged, 80 and over
dc.subject.meshDNA
dc.subject.meshDNA Damage
dc.subject.meshDeoxyguanosine
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshLymphocytes
dc.subject.meshMale
dc.subject.meshMiddle Aged
dc.subject.meshSmoking
dc.titleNo association between N7-methyldeoxyguanosine and 8-oxodeoxyguanosine levels in human lymphocyte DNA.en
dc.typeArticleen
dc.contributor.departmentCentre for Occupational and Environmental Health, Division of Epidemiology and Health Sciences, Medical School, University of Manchester, Oxford Road, Manchester, M13 9PT, UK.en
dc.identifier.journalMutation Researchen
html.description.abstractTo examine associations between two different classes of DNA damage that can occur through endogenous processes or exogenous exposures such as smoking, N7-methyldeoxyguanosine (N7-MedG) and 8-oxodeoxyguanosine (8-oxodG) levels were measured in lymphocyte DNA from 22 bronchoscopy patients. 8-OxodG and N7-MedG was detected in 100% and 91% of samples, respectively with 8-oxodG levels being approx 20 times higher (mean 8.39+/-3.578-oxodG/10(6)dG versus 0.41+/-0.33 N7-MedG/10(6) dG) which provides an indication of the relative importance of the agents that induce oxidative DNA damage or alkylation damage. The sources of these genotoxic lesions remain to be established but N7-MedG and 8-oxodG levels were not correlated (r(2)<0.01) suggesting that there is no association between alkylating agent and reactive oxygen species exposure, their metabolism and/or the DNA repair processes that can remove this DNA damage.


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