No association between N7-methyldeoxyguanosine and 8-oxodeoxyguanosine levels in human lymphocyte DNA.
Authors
Harrison, Kathryn LCrosbie, Philip A J
Agius, Raymond M
Barber, Philip V
Carus, Mark
Margison, Geoffrey P
Povey, Andrew C
Affiliation
Centre for Occupational and Environmental Health, Division of Epidemiology and Health Sciences, Medical School, University of Manchester, Oxford Road, Manchester, M13 9PT, UK.Issue Date
2006-08-30
Metadata
Show full item recordAbstract
To examine associations between two different classes of DNA damage that can occur through endogenous processes or exogenous exposures such as smoking, N7-methyldeoxyguanosine (N7-MedG) and 8-oxodeoxyguanosine (8-oxodG) levels were measured in lymphocyte DNA from 22 bronchoscopy patients. 8-OxodG and N7-MedG was detected in 100% and 91% of samples, respectively with 8-oxodG levels being approx 20 times higher (mean 8.39+/-3.578-oxodG/10(6)dG versus 0.41+/-0.33 N7-MedG/10(6) dG) which provides an indication of the relative importance of the agents that induce oxidative DNA damage or alkylation damage. The sources of these genotoxic lesions remain to be established but N7-MedG and 8-oxodG levels were not correlated (r(2)<0.01) suggesting that there is no association between alkylating agent and reactive oxygen species exposure, their metabolism and/or the DNA repair processes that can remove this DNA damage.Citation
No association between N7-methyldeoxyguanosine and 8-oxodeoxyguanosine levels in human lymphocyte DNA. 2006, 600 (1-2):125-30 Mutat. Res.Journal
Mutation ResearchDOI
10.1016/j.mrfmmm.2006.03.005PubMed ID
16765387Type
ArticleLanguage
enISSN
0027-5107ae974a485f413a2113503eed53cd6c53
10.1016/j.mrfmmm.2006.03.005
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