• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsProfilesView

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    GINS maintains association of Cdc45 with MCM in replisome progression complexes at eukaryotic DNA replication forks.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Gambus, Agnieszka
    Jones, Richard C
    Sanchez-Diaz, Alberto
    Kanemaki, Masato
    Van Deursen, Frederick
    Edmondson, Ricky D
    Labib, Karim
    Affiliation
    Cancer Research UK, Paterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Manchester, M20 4BX, UK.
    Issue Date
    2006-04
    
    Metadata
    Show full item record
    Abstract
    The components of the replisome that preserve genomic stability by controlling the progression of eukaryotic DNA replication forks are poorly understood. Here, we show that the GINS (go ichi ni san) complex allows the MCM (minichromosome maintenance) helicase to interact with key regulatory proteins in large replisome progression complexes (RPCs) that are assembled during initiation and disassembled at the end of S phase. RPC components include the essential initiation and elongation factor, Cdc45, the checkpoint mediator Mrc1, the Tof1-Csm3 complex that allows replication forks to pause at protein-DNA barriers, the histone chaperone FACT (facilitates chromatin transcription) and Ctf4, which helps to establish sister chromatid cohesion. RPCs also interact with Mcm10 and topoisomerase I. During initiation, GINS is essential for a specific subset of RPC proteins to interact with MCM. GINS is also important for the normal progression of DNA replication forks, and we show that it is required after initiation to maintain the association between MCM and Cdc45 within RPCs.
    Citation
    GINS maintains association of Cdc45 with MCM in replisome progression complexes at eukaryotic DNA replication forks. 2006, 8 (4):358-66 Nat. Cell Biol.
    Journal
    Nature Cell Biology
    URI
    http://hdl.handle.net/10541/73113
    DOI
    10.1038/ncb1382
    PubMed ID
    16531994
    Type
    Article
    Language
    en
    ISSN
    1465-7392
    ae974a485f413a2113503eed53cd6c53
    10.1038/ncb1382
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • Molecular anatomy and regulation of a stable replisome at a paused eukaryotic DNA replication fork.
    • Authors: Calzada A, Hodgson B, Kanemaki M, Bueno A, Labib K
    • Issue date: 2005 Aug 15
    • Dpb2 integrates the leading-strand DNA polymerase into the eukaryotic replisome.
    • Authors: Sengupta S, van Deursen F, de Piccoli G, Labib K
    • Issue date: 2013 Apr 8
    • Ctf4 coordinates the progression of helicase and DNA polymerase alpha.
    • Authors: Tanaka H, Katou Y, Yagura M, Saitoh K, Itoh T, Araki H, Bando M, Shirahige K
    • Issue date: 2009 Jul
    • Mcm10 plays a role in functioning of the eukaryotic replicative DNA helicase, Cdc45-Mcm-GINS.
    • Authors: Watase G, Takisawa H, Kanemaki MT
    • Issue date: 2012 Feb 21
    • Distinct roles for Sld3 and GINS during establishment and progression of eukaryotic DNA replication forks.
    • Authors: Kanemaki M, Labib K
    • Issue date: 2006 Apr 19
    DSpace software (copyright © 2002 - 2025)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.