Comparison of doxycycline delivery methods for Tet-inducible gene expression in a subcutaneous xenograft model.
AffiliationCancer Research UK, Paterson Institute for Cancer Research, University of Manchester, Manchester, UK.
MetadataShow full item record
AbstractDoxycycline (Dox) controlled Tet systems provide a powerful and commonly used method for functional studies on the consequences of gene overexpression/downregulation. However, whereas Dox delivery in tissue culture in vitro is relatively simple, the situation in vivo is more complex. Several methods of Dox delivery in vivo have been described-e.g., in drinking water containing alcohol, in drinking water containing various concentrations of sucrose, and in feed. Unfortunately there are no reports directly comparing the advantages and disadvantages of these diverse methods, and there is no generally accepted standard. We therefore compared four non-invasive methods of Dox delivery in vivo-in drinking water, by gavage, as a jelly, and in standard feed. To assess the delivery of Dox by these methods, we used a subcutaneous xenograft model based on colorectal carcinoma cells engineered for Dox-inducible expression of an activated mutant of c-Src and the luciferase reporter gene. Our results indicate that feed represents the most favorable method of Dox administration.
CitationComparison of doxycycline delivery methods for Tet-inducible gene expression in a subcutaneous xenograft model. 2007, 18 (2):120-3 J Biomol Tech
JournalJournal of Biomolecular Techniques
- Anti-invasion and anti-tumor growth effect of doxycycline treatment for human oral squamous-cell carcinoma--in vitro and in vivo studies.
- Authors: Shen LC, Chen YK, Lin LM, Shaw SY
- Issue date: 2010 Mar
- 4-Epidoxycycline: an alternative to doxycycline to control gene expression in conditional mouse models.
- Authors: Eger K, Hermes M, Uhlemann K, Rodewald S, Ortwein J, Brulport M, Bauer AW, Schormann W, Lupatsch F, Schiffer IB, Heimerdinger CK, Gebhard S, Spangenberg C, Prawitt D, Trost T, Zabel B, Sauer C, Tanner B, Kolbl H, Krugel U, Franke H, Illes P, Madaj-Sterba P, Bockamp EO, Beckers T, Hengstler JG
- Issue date: 2004 Oct 22
- Serum and tissue concentrations of doxycycline in broilers after the sub-cutaneous injection of a long-acting formulation.
- Authors: Gutiérrez L, Vargas-Estrada D, Rosario C, Sumano H
- Issue date: 2012
- Increases in c-Src expression level and activity do not promote the growth of human colorectal carcinoma cells in vitro and in vivo.
- Authors: Welman A, Cawthorne C, Ponce-Perez L, Barraclough J, Danson S, Murray S, Cummings J, Allen TD, Dive C
- Issue date: 2006 Nov
- Recombinant AAV-mediated HSVtk gene transfer with direct intratumoral injections and Tet-On regulation for implanted human breast cancer.
- Authors: Li ZB, Zeng ZJ, Chen Q, Luo SQ, Hu WX
- Issue date: 2006 Mar 16