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dc.contributor.authorBibi, Rufzan
dc.contributor.authorPranesh, Nagarajan
dc.contributor.authorSaunders, Mark P
dc.contributor.authorWilson, Malcolm S
dc.contributor.authorO'Dwyer, Sarah T
dc.contributor.authorStern, Peter L
dc.contributor.authorRenehan, Andrew G
dc.date.accessioned2009-07-07T15:53:18Z
dc.date.available2009-07-07T15:53:18Z
dc.date.issued2006-11-06
dc.identifier.citationA specific cadherin phenotype may characterise the disseminating yet non-metastatic behaviour of pseudomyxoma peritonei. 2006, 95 (9):1258-64 Br. J. Canceren
dc.identifier.issn0007-0920
dc.identifier.pmid17031402
dc.identifier.doi10.1038/sj.bjc.6603398
dc.identifier.urihttp://hdl.handle.net/10541/72868
dc.description.abstractPseudomyxoma peritonei (PMP) is a rare neoplasm of mainly appendiceal origin, characterised by excess intra-abdominal mucin production leading to high morbidity and mortality. While histological features are frequently indolent, this tumour disseminates aggressively throughout the abdominal cavity, yet seldom metastasises. This study determined the expression of several markers of colorectal differentiation (carcinoembryonic antigen (CEA), cytokeratins (CK20 and CK7), epithelial membrane antigen), mucin production (MUC-2, interleukin-9 (IL-9), IL-9 receptor (IL-9Ralpha)), and cell adhesion (N- and E-cadherin, vimentin) in PMP tissue (n=26) compared with expressions in normal colonic mucosa (n=19) and colorectal adenocarcinoma (n=26). Expressions of CEA and cytokeratins were similar for PMP as those in colorectal adenocarcinomas with the exception that the CK20-/CK7- pattern was rare in PMP (Fisher's exact test: P=0.001). Similarly, expressions of mucin-related proteins were comparable for adenocarcinoma and PMP, with the exception that IL-9 expression was uncommon in adenocarcinoma (P=0.009). Pseudomyxoma peritonei demonstrated a specific pattern of adhesion-related protein expressions of increased N-cadherin, reduced E-cadherin, and increased vimentin (P=0.004), a phenotype suggesting a possible epithelial-mesenchymal transition state. Primary PMP cell cultures were successfully maintained and demonstrated marker expressions similar to those seen in in vivo tissues. These early characterisation studies demonstrate similarities between PMP and colorectal adenocarcinoma, but also reveal a specific cadherin phenotype that may characterise the distinct non-metastasising behaviour of PMP, and form the basis for future mechanistic and therapy-targeting research.
dc.language.isoenen
dc.subjectCancer Metastasisen
dc.subjectPeritoneal Canceren
dc.subjectTumour Cellsen
dc.subject.meshAdult
dc.subject.meshAged
dc.subject.meshAged, 80 and over
dc.subject.meshCadherins
dc.subject.meshCarcinoembryonic Antigen
dc.subject.meshDiagnosis, Differential
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshImmunohistochemistry
dc.subject.meshInterleukin-9
dc.subject.meshKeratin-20
dc.subject.meshKeratin-7
dc.subject.meshMale
dc.subject.meshMiddle Aged
dc.subject.meshMucin-1
dc.subject.meshMucin-2
dc.subject.meshMucins
dc.subject.meshNeoplasm Metastasis
dc.subject.meshPeritoneal Neoplasms
dc.subject.meshPseudomyxoma Peritonei
dc.subject.meshReceptors, Interleukin-9
dc.subject.meshTumor Cells, Cultured
dc.subject.meshVimentin
dc.titleA specific cadherin phenotype may characterise the disseminating yet non-metastatic behaviour of pseudomyxoma peritonei.en
dc.typeArticleen
dc.contributor.departmentCancer Research UK Immunology Group, Paterson Institute for Cancer Research, Manchester, UK.en
dc.identifier.journalBritish Journal of Canceren
html.description.abstractPseudomyxoma peritonei (PMP) is a rare neoplasm of mainly appendiceal origin, characterised by excess intra-abdominal mucin production leading to high morbidity and mortality. While histological features are frequently indolent, this tumour disseminates aggressively throughout the abdominal cavity, yet seldom metastasises. This study determined the expression of several markers of colorectal differentiation (carcinoembryonic antigen (CEA), cytokeratins (CK20 and CK7), epithelial membrane antigen), mucin production (MUC-2, interleukin-9 (IL-9), IL-9 receptor (IL-9Ralpha)), and cell adhesion (N- and E-cadherin, vimentin) in PMP tissue (n=26) compared with expressions in normal colonic mucosa (n=19) and colorectal adenocarcinoma (n=26). Expressions of CEA and cytokeratins were similar for PMP as those in colorectal adenocarcinomas with the exception that the CK20-/CK7- pattern was rare in PMP (Fisher's exact test: P=0.001). Similarly, expressions of mucin-related proteins were comparable for adenocarcinoma and PMP, with the exception that IL-9 expression was uncommon in adenocarcinoma (P=0.009). Pseudomyxoma peritonei demonstrated a specific pattern of adhesion-related protein expressions of increased N-cadherin, reduced E-cadherin, and increased vimentin (P=0.004), a phenotype suggesting a possible epithelial-mesenchymal transition state. Primary PMP cell cultures were successfully maintained and demonstrated marker expressions similar to those seen in in vivo tissues. These early characterisation studies demonstrate similarities between PMP and colorectal adenocarcinoma, but also reveal a specific cadherin phenotype that may characterise the distinct non-metastasising behaviour of PMP, and form the basis for future mechanistic and therapy-targeting research.


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