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    A parallel proteomic and metabolomic analysis of the hydrogen peroxide- and Sty1p-dependent stress response in Schizosaccharomyces pombe.

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    Authors
    Weeks, Mark E
    Sinclair, John
    Butt, Amna
    Chung, Yuen-Li
    Worthington, Jessica L
    Wilkinson, Caroline R M
    Griffiths, John R
    Jones, Nic
    Waterfield, Michael D
    Timms, John F
    Affiliation
    Ludwig Institute for Cancer Research, University College London, Cruciform Building, London, UK.
    Issue Date
    2006-05
    
    Metadata
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    Abstract
    Using an integrated approach incorporating proteomics, metabolomics and published mRNA data, we have investigated the effects of hydrogen peroxide on wild type and a Sty1p-deletion mutant of the fission yeast Schizosaccharomyces pombe. Differential protein expression analysis based on the modification of proteins with matched fluorescent labelling reagents (2-D-DIGE) is the foundation of the quantitative proteomics approach. This study identifies 260 differentially expressed protein isoforms from 2-D-DIGE gels using MALDI MS and reveals the complexity of the cellular response to oxidative stress and the dependency on the Sty1p stress-activated protein kinase. We show the relationship between these protein changes and mRNA expression levels identified in a parallel whole genome study, and discuss the regulatory mechanisms involved in protecting cells against hydrogen peroxide and the involvement of Sty1p-dependent stress-activated protein kinase signalling. Metabolomic profiling of 29 intermediates using 1H NMR was also conducted alongside the protein analysis using the same sample sets, allowing examination of how the protein changes might affect the metabolic pathways and biological processes involved in the oxidative stress response. This combined analysis identifies a number of interlinked metabolic pathways that exhibit stress- and Sty1-dependent patterns of regulation.
    Citation
    A parallel proteomic and metabolomic analysis of the hydrogen peroxide- and Sty1p-dependent stress response in Schizosaccharomyces pombe. 2006, 6 (9):2772-96 Proteomics
    Journal
    Proteomics
    URI
    http://hdl.handle.net/10541/72676
    DOI
    10.1002/pmic.200500741
    PubMed ID
    16548067
    Type
    Article
    Language
    en
    ISSN
    1615-9853
    ae974a485f413a2113503eed53cd6c53
    10.1002/pmic.200500741
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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