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    Proteomic response of Schizosaccharomyces pombe to static and oscillating extremely low-frequency electromagnetic fields.

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    Authors
    Sinclair, John
    Weeks, Mark E
    Butt, Amna
    Worthington, Jessica L
    Akpan, Akunna
    Jones, Nic
    Waterfield, Michael D
    Allan, Donald
    Timms, John F
    Affiliation
    Ludwig Institute for Cancer Research, Department of Biochemistry and Molecular Biology University College London, UK.
    Issue Date
    2006-09
    
    Metadata
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    Abstract
    There is considerable public concern regarding the health effects of exposure to low-frequency electromagnetic fields. In addition, the association between exposure and disease incidence or the possible biological effects of exposure are unclear. Using 2D-DIGE and MS in a blind study, we have investigated the effects of static and oscillating extremely low-frequency electromagnetic fields (ELF EMFs) on the proteomes of wild type Schizosaccharomyces pombe and a Sty1p deletion mutant which displays increased sensitivity to a variety of cellular stresses. Whilst this study identifies a number of protein isoforms that display significant differential expression across experimental conditions, there was no correlation between their patterns of expression and the ELF EMF exposure regimen. We conclude that there are no significant effects of either static or oscillating EMF on the yeast proteome at the sensitivity afforded by 2D-DIGE. We hypothesise that the proteins identified must be sensitive to subtle changes in culture and/or handling conditions, and that the identification of these proteins in other proteomic studies should be treated with some caution when the results of such studies are interpreted in a biological context.
    Citation
    Proteomic response of Schizosaccharomyces pombe to static and oscillating extremely low-frequency electromagnetic fields. 2006, 6 (17):4755-64 Proteomics
    Journal
    Proteomics
    URI
    http://hdl.handle.net/10541/72675
    DOI
    10.1002/pmic.200500861
    PubMed ID
    16897687
    Type
    Article
    Language
    en
    ISSN
    1615-9853
    ae974a485f413a2113503eed53cd6c53
    10.1002/pmic.200500861
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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