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dc.contributor.authorWang, Qiuyu
dc.contributor.authorKumar, Shant
dc.contributor.authorSlevin, Mark
dc.contributor.authorKumar, Patricia
dc.date.accessioned2009-07-06T11:46:16Z
dc.date.available2009-07-06T11:46:16Z
dc.date.issued2006-09-01
dc.identifier.citationFunctional analysis of alternative isoforms of the transcription factor PAX3 in melanocytes in vitro. 2006, 66 (17):8574-80 Cancer Res.en
dc.identifier.issn1538-7445
dc.identifier.pmid16951170
dc.identifier.doi10.1158/0008-5472.CAN-06-0947
dc.identifier.urihttp://hdl.handle.net/10541/72578
dc.description.abstractTranscription factor PAX3 has seven isoforms of which PAX3c has been studied extensively whereas the functions of the other isoforms are less well known. Here, we found that PAX3 isoforms in a stable transfection system have different biological functions in mouse melanocytes in vitro. PAX3a and PAX3b had negative effects on melanocyte proliferation but had no discernable effect on melanocyte growth in soft agar. PAX3a did not affect cell migration and apoptosis but PAX3b reduced migration and accelerated apoptosis. PAX3c and PAX3d promoted cell proliferation, migration, transformation, and survival. PAX3e reduced melanocyte growth; transformation and migration were unchanged and apoptosis was increased in vitro. PAX3g did not influence cell proliferation or apoptosis. Cells expressing PAX3g were able to grow in soft agar but migration was reduced. PAX3h increased cell proliferation, migration, survival, and transformation. These functional studies have advanced our understanding of the effects of PAX3 isoforms in melanocytes and their potential contribution in tumorigenesis.
dc.language.isoenen
dc.subjectCell Line Tumouren
dc.subjectCancer RNAen
dc.subject.meshCell Cycle
dc.subject.meshCell Division
dc.subject.meshCell Line, Tumor
dc.subject.meshCell Movement
dc.subject.meshEmbryonic Development
dc.subject.meshHumans
dc.subject.meshMelanocytes
dc.subject.meshMelanoma
dc.subject.meshNeural Crest
dc.subject.meshPaired Box Transcription Factors
dc.subject.meshPromoter Regions, Genetic
dc.subject.meshProtein Isoforms
dc.subject.meshRNA, Neoplasm
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
dc.titleFunctional analysis of alternative isoforms of the transcription factor PAX3 in melanocytes in vitro.en
dc.typeArticleen
dc.contributor.departmentSchool of Biology, Chemistry and Health Science, Manchester Metropolitan University, Manchester, United Kingdom.en
dc.identifier.journalCancer Researchen
html.description.abstractTranscription factor PAX3 has seven isoforms of which PAX3c has been studied extensively whereas the functions of the other isoforms are less well known. Here, we found that PAX3 isoforms in a stable transfection system have different biological functions in mouse melanocytes in vitro. PAX3a and PAX3b had negative effects on melanocyte proliferation but had no discernable effect on melanocyte growth in soft agar. PAX3a did not affect cell migration and apoptosis but PAX3b reduced migration and accelerated apoptosis. PAX3c and PAX3d promoted cell proliferation, migration, transformation, and survival. PAX3e reduced melanocyte growth; transformation and migration were unchanged and apoptosis was increased in vitro. PAX3g did not influence cell proliferation or apoptosis. Cells expressing PAX3g were able to grow in soft agar but migration was reduced. PAX3h increased cell proliferation, migration, survival, and transformation. These functional studies have advanced our understanding of the effects of PAX3 isoforms in melanocytes and their potential contribution in tumorigenesis.


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