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    The fate of human Langerhans cells in hematopoietic stem cell transplantation.

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    Authors
    Collin, Matthew P
    Hart, Derek N J
    Jackson, Graham H
    Cook, Gordon
    Cavet, James
    Mackinnon, Stephen
    Middleton, Peter G
    Dickinson, Anne M
    Affiliation
    Haematological Sciences, University of Newcastle, Newcastle upon Tyne NE2 4HH, England, UK. matthew.collin@ncl.ac.uk
    Issue Date
    2006-01-23
    
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    Abstract
    Langerhans cells (LC) and other antigen-presenting cells are believed to be critical in initiating graft versus host responses that influence the outcome of allogeneic hematopoietic stem cell transplantation. However, their fate in humans is poorly understood. We have sought to define the effect of conditioning regimes and graft versus host disease (GVHD) on the survival of recipient LC and reconstitution of donor cells after transplant. Confocal microscopy of epidermal sheets shows that full intensity transplant (FIT) depletes LC more rapidly than reduced intensity transplant (RIT) at day 0, although the nadir is similar in both at 14-21 d. Recovery occurs rapidly within 40 d in the absence of acute GVHD, but is delayed beyond 100 d when GVHD is active. LC chimerism was determined in sex-mismatched transplants using a two-step Giemsa/fluorescence in situ hybridization assay on isolated cells. Acquisition of donor chimerism at 40 d is more rapid after FIT (97%) than RIT (36.5%), irrespective of blood myeloid engraftment. At 100 d, all transplants achieve at least 90% LC donor chimerism and over half achieve 100%. Complete donor chimerism is associated with prior acute cutaneous GVHD, suggesting a role for allogeneic T cells in promoting LC engraftment.
    Citation
    The fate of human Langerhans cells in hematopoietic stem cell transplantation. 2006, 203 (1):27-33 J. Exp. Med.
    Journal
    The Journal of Experimental Medicine
    URI
    http://hdl.handle.net/10541/72416
    DOI
    10.1084/jem.20051787
    PubMed ID
    16390938
    Type
    Article
    Language
    en
    ISSN
    0022-1007
    ae974a485f413a2113503eed53cd6c53
    10.1084/jem.20051787
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