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dc.contributor.authorLi, Hong Gui
dc.contributor.authorWang, Qiuyu
dc.contributor.authorHong, Mei Li
dc.contributor.authorKumar, Shant
dc.contributor.authorParker, Craig
dc.contributor.authorSlevin, Mark
dc.contributor.authorKumar, Patricia
dc.date.accessioned2009-06-30T14:08:21Z
dc.date.available2009-06-30T14:08:21Z
dc.date.issued2007-08-18
dc.identifier.citationPAX3 and PAX3-FKHR promote rhabdomyosarcoma cell survival through downregulation of PTEN. 2007, 253 (2):215-23 Cancer Lett.en
dc.identifier.issn0304-3835
dc.identifier.pmid17350164
dc.identifier.doi10.1016/j.canlet.2007.01.020
dc.identifier.urihttp://hdl.handle.net/10541/71956
dc.description.abstractPAX3 or PAX3-FKHR expression is implicated in cell transformation and tumourigenesis. Here, C2C12 myoblasts were transfected with a sense Pax3 vector and a pTet-On system to induce Pax3 expression, whereas to downregulate PAX3-FKHR, Rh18 was transfected with an antisense Pax3 with a pTet-On system. The inhibition of PAX3-FKHR in Rh18 induced upregulation of PTEN. Decreased resistance to apoptosis and increased transformation ability were observed in the Rh18 cells with PAX3-FKHR downregulation. Conversely, Pax3 induction in C2C12 cells downregulated the expression of PTEN and p27(Kip1). These results indicate that the involvement of PAX3 and PAX3-FKHR in rhabdomyosarcoma tumourigenesis may be through downregulation of PTEN tumour suppressor gene, affecting the PTEN/AKT survival pathway.
dc.language.isoenen
dc.subjectCell Line Tumouren
dc.subjectMuscle Canceren
dc.subject.meshAnimals
dc.subject.meshCell Line, Tumor
dc.subject.meshCell Survival
dc.subject.meshCell Transformation, Neoplastic
dc.subject.meshChromatin Immunoprecipitation
dc.subject.meshCyclin-Dependent Kinase Inhibitor p27
dc.subject.meshDown-Regulation
dc.subject.meshDoxycycline
dc.subject.meshGene Expression Regulation, Neoplastic
dc.subject.meshHumans
dc.subject.meshMice
dc.subject.meshNeoplasms, Muscle Tissue
dc.subject.meshOncogene Proteins, Fusion
dc.subject.meshPTEN Phosphohydrolase
dc.subject.meshPaired Box Transcription Factors
dc.subject.meshPromoter Regions, Genetic
dc.subject.meshRhabdomyosarcoma
dc.titlePAX3 and PAX3-FKHR promote rhabdomyosarcoma cell survival through downregulation of PTEN.en
dc.typeArticleen
dc.contributor.departmentSchool of Biology, Chemistry and Health Science, Manchester Metropolitan University, Manchester M1 5GD, United Kingdom.en
dc.identifier.journalCancer Lettersen
html.description.abstractPAX3 or PAX3-FKHR expression is implicated in cell transformation and tumourigenesis. Here, C2C12 myoblasts were transfected with a sense Pax3 vector and a pTet-On system to induce Pax3 expression, whereas to downregulate PAX3-FKHR, Rh18 was transfected with an antisense Pax3 with a pTet-On system. The inhibition of PAX3-FKHR in Rh18 induced upregulation of PTEN. Decreased resistance to apoptosis and increased transformation ability were observed in the Rh18 cells with PAX3-FKHR downregulation. Conversely, Pax3 induction in C2C12 cells downregulated the expression of PTEN and p27(Kip1). These results indicate that the involvement of PAX3 and PAX3-FKHR in rhabdomyosarcoma tumourigenesis may be through downregulation of PTEN tumour suppressor gene, affecting the PTEN/AKT survival pathway.


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