PAX3 and PAX3-FKHR promote rhabdomyosarcoma cell survival through downregulation of PTEN.
Affiliation
School of Biology, Chemistry and Health Science, Manchester Metropolitan University, Manchester M1 5GD, United Kingdom.Issue Date
2007-08-18
Metadata
Show full item recordAbstract
PAX3 or PAX3-FKHR expression is implicated in cell transformation and tumourigenesis. Here, C2C12 myoblasts were transfected with a sense Pax3 vector and a pTet-On system to induce Pax3 expression, whereas to downregulate PAX3-FKHR, Rh18 was transfected with an antisense Pax3 with a pTet-On system. The inhibition of PAX3-FKHR in Rh18 induced upregulation of PTEN. Decreased resistance to apoptosis and increased transformation ability were observed in the Rh18 cells with PAX3-FKHR downregulation. Conversely, Pax3 induction in C2C12 cells downregulated the expression of PTEN and p27(Kip1). These results indicate that the involvement of PAX3 and PAX3-FKHR in rhabdomyosarcoma tumourigenesis may be through downregulation of PTEN tumour suppressor gene, affecting the PTEN/AKT survival pathway.Citation
PAX3 and PAX3-FKHR promote rhabdomyosarcoma cell survival through downregulation of PTEN. 2007, 253 (2):215-23 Cancer Lett.Journal
Cancer LettersDOI
10.1016/j.canlet.2007.01.020PubMed ID
17350164Type
ArticleLanguage
enISSN
0304-3835ae974a485f413a2113503eed53cd6c53
10.1016/j.canlet.2007.01.020
Scopus Count
Collections
Related articles
- The PAX3-FKHR fusion gene of rhabdomyosarcoma cooperates with loss of p16INK4A to promote bypass of cellular senescence.
- Authors: Linardic CM, Naini S, Herndon JE 2nd, Kesserwan C, Qualman SJ, Counter CM
- Issue date: 2007 Jul 15
- PAX3-FKHR transformation increases 26 S proteasome-dependent degradation of p27Kip1, a potential role for elevated Skp2 expression.
- Authors: Zhang L, Wang C
- Issue date: 2003 Jan 3
- PAX3-FOXO1 is essential for tumour initiation and maintenance but not recurrence in a human myoblast model of rhabdomyosarcoma.
- Authors: Pandey PR, Chatterjee B, Olanich ME, Khan J, Miettinen MM, Hewitt SM, Barr FG
- Issue date: 2017 Apr
- Insulin-like growth factor II and PAX3-FKHR cooperate in the oncogenesis of rhabdomyosarcoma.
- Authors: Wang W, Kumar P, Wang W, Epstein J, Helman L, Moore JV, Kumar S
- Issue date: 1998 Oct 1
- Regulation of expression of stromal-derived factor-1 receptors: CXCR4 and CXCR7 in human rhabdomyosarcomas.
- Authors: Tarnowski M, Grymula K, Reca R, Jankowski K, Maksym R, Tarnowska J, Przybylski G, Barr FG, Kucia M, Ratajczak MZ
- Issue date: 2010 Jan