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A microarray study of gene and protein regulation in human and rat brain following middle cerebral artery occlusion.

dc.contributor.authorMitsios, Nick
dc.contributor.authorSaka, Mohamad
dc.contributor.authorKrupinski, Jerzy
dc.contributor.authorPennucci, Roberta
dc.contributor.authorSanfeliu, Coral
dc.contributor.authorWang, Qiuyu
dc.contributor.authorRubio, Francisco
dc.contributor.authorGaffney, John
dc.contributor.authorKumar, Patricia
dc.contributor.authorKumar, Shant
dc.contributor.authorSullivan, Matthew
dc.contributor.authorSlevin, Mark
dc.date.accessioned2009-06-30T11:40:03Z
dc.date.available2009-06-30T11:40:03Z
dc.date.issued2007-11
dc.identifier.citationA microarray study of gene and protein regulation in human and rat brain following middle cerebral artery occlusion. 2007, 8:93 BMC Neuroscien
dc.identifier.issn1471-2202
dc.identifier.pmid17997827
dc.identifier.doi10.1186/1471-2202-8-93
dc.identifier.urihttp://hdl.handle.net/10541/71922
dc.description.abstractBACKGROUND: Altered gene expression is an important feature of ischemic cerebral injury and affects proteins of many functional classes. We have used microarrays to investigate the changes in gene expression at various times after middle cerebral artery occlusion in human and rat brain. RESULTS: Our results demonstrated a significant difference in the number of genes affected and the time-course of expression between the two cases. The total number of deregulated genes in the rat was 335 versus 126 in the human, while, of 393 overlapping genes between the two array sets, 184 were changed only in the rat and 36 in the human with a total of 41 genes deregulated in both cases. Interestingly, the mean fold changes were much higher in the human. The expression of novel genes, including p21-activated kinase 1 (PAK1), matrix metalloproteinase 11 (MMP11) and integrase interactor 1, was further analyzed by RT-PCR, Western blotting and immunohistochemistry. Strong neuronal staining was seen for PAK1 and MMP11. CONCLUSION: Our findings confirmed previous studies reporting that gene expression screening can detect known and unknown transcriptional features of stroke and highlight the importance of research using human brain tissue in the search for novel therapeutic agents.
dc.language.isoenen
dc.subjectFoetusen
dc.subject.meshAged
dc.subject.meshAged, 80 and over
dc.subject.meshAnimals
dc.subject.meshAnoxia
dc.subject.meshBrain
dc.subject.meshCells, Cultured
dc.subject.meshChromosomal Proteins, Non-Histone
dc.subject.meshDNA-Binding Proteins
dc.subject.meshFemale
dc.subject.meshFetus
dc.subject.meshGene Expression Regulation
dc.subject.meshGlucose
dc.subject.meshHumans
dc.subject.meshInfarction, Middle Cerebral Artery
dc.subject.meshMale
dc.subject.meshMatrix Metalloproteinase 11
dc.subject.meshMiddle Aged
dc.subject.meshOligonucleotide Array Sequence Analysis
dc.subject.meshRats
dc.subject.meshRats, Sprague-Dawley
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
dc.subject.meshStroke
dc.subject.meshTranscription Factors
dc.subject.meshp21-Activated Kinases
dc.titleA microarray study of gene and protein regulation in human and rat brain following middle cerebral artery occlusion.en
dc.typeArticleen
dc.contributor.departmentSchool of Biology, Chemistry and Health Science, John Dalton Building, Manchester Metropolitan University, Chester Street, Manchester, UK. n.mitsios@mmu.ac.uken
dc.identifier.journalBMC Neuroscienceen
html.description.abstractBACKGROUND: Altered gene expression is an important feature of ischemic cerebral injury and affects proteins of many functional classes. We have used microarrays to investigate the changes in gene expression at various times after middle cerebral artery occlusion in human and rat brain. RESULTS: Our results demonstrated a significant difference in the number of genes affected and the time-course of expression between the two cases. The total number of deregulated genes in the rat was 335 versus 126 in the human, while, of 393 overlapping genes between the two array sets, 184 were changed only in the rat and 36 in the human with a total of 41 genes deregulated in both cases. Interestingly, the mean fold changes were much higher in the human. The expression of novel genes, including p21-activated kinase 1 (PAK1), matrix metalloproteinase 11 (MMP11) and integrase interactor 1, was further analyzed by RT-PCR, Western blotting and immunohistochemistry. Strong neuronal staining was seen for PAK1 and MMP11. CONCLUSION: Our findings confirmed previous studies reporting that gene expression screening can detect known and unknown transcriptional features of stroke and highlight the importance of research using human brain tissue in the search for novel therapeutic agents.


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