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    A microarray study of gene and protein regulation in human and rat brain following middle cerebral artery occlusion.

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    Authors
    Mitsios, Nick
    Saka, Mohamad
    Krupinski, Jerzy
    Pennucci, Roberta
    Sanfeliu, Coral
    Wang, Qiuyu
    Rubio, Francisco
    Gaffney, John
    Kumar, Patricia
    Kumar, Shant
    Sullivan, Matthew
    Slevin, Mark
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    Affiliation
    School of Biology, Chemistry and Health Science, John Dalton Building, Manchester Metropolitan University, Chester Street, Manchester, UK. n.mitsios@mmu.ac.uk
    Issue Date
    2007-11
    
    Metadata
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    Abstract
    BACKGROUND: Altered gene expression is an important feature of ischemic cerebral injury and affects proteins of many functional classes. We have used microarrays to investigate the changes in gene expression at various times after middle cerebral artery occlusion in human and rat brain. RESULTS: Our results demonstrated a significant difference in the number of genes affected and the time-course of expression between the two cases. The total number of deregulated genes in the rat was 335 versus 126 in the human, while, of 393 overlapping genes between the two array sets, 184 were changed only in the rat and 36 in the human with a total of 41 genes deregulated in both cases. Interestingly, the mean fold changes were much higher in the human. The expression of novel genes, including p21-activated kinase 1 (PAK1), matrix metalloproteinase 11 (MMP11) and integrase interactor 1, was further analyzed by RT-PCR, Western blotting and immunohistochemistry. Strong neuronal staining was seen for PAK1 and MMP11. CONCLUSION: Our findings confirmed previous studies reporting that gene expression screening can detect known and unknown transcriptional features of stroke and highlight the importance of research using human brain tissue in the search for novel therapeutic agents.
    Citation
    A microarray study of gene and protein regulation in human and rat brain following middle cerebral artery occlusion. 2007, 8:93 BMC Neurosci
    Journal
    BMC Neuroscience
    URI
    http://hdl.handle.net/10541/71922
    DOI
    10.1186/1471-2202-8-93
    PubMed ID
    17997827
    Type
    Article
    Language
    en
    ISSN
    1471-2202
    ae974a485f413a2113503eed53cd6c53
    10.1186/1471-2202-8-93
    Scopus Count
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