A microarray study of gene and protein regulation in human and rat brain following middle cerebral artery occlusion.
Authors
Mitsios, NickSaka, Mohamad
Krupinski, Jerzy
Pennucci, Roberta
Sanfeliu, Coral
Wang, Qiuyu
Rubio, Francisco
Gaffney, John
Kumar, Patricia
Kumar, Shant
Sullivan, Matthew
Slevin, Mark
Affiliation
School of Biology, Chemistry and Health Science, John Dalton Building, Manchester Metropolitan University, Chester Street, Manchester, UK. n.mitsios@mmu.ac.ukIssue Date
2007-11
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BACKGROUND: Altered gene expression is an important feature of ischemic cerebral injury and affects proteins of many functional classes. We have used microarrays to investigate the changes in gene expression at various times after middle cerebral artery occlusion in human and rat brain. RESULTS: Our results demonstrated a significant difference in the number of genes affected and the time-course of expression between the two cases. The total number of deregulated genes in the rat was 335 versus 126 in the human, while, of 393 overlapping genes between the two array sets, 184 were changed only in the rat and 36 in the human with a total of 41 genes deregulated in both cases. Interestingly, the mean fold changes were much higher in the human. The expression of novel genes, including p21-activated kinase 1 (PAK1), matrix metalloproteinase 11 (MMP11) and integrase interactor 1, was further analyzed by RT-PCR, Western blotting and immunohistochemistry. Strong neuronal staining was seen for PAK1 and MMP11. CONCLUSION: Our findings confirmed previous studies reporting that gene expression screening can detect known and unknown transcriptional features of stroke and highlight the importance of research using human brain tissue in the search for novel therapeutic agents.Citation
A microarray study of gene and protein regulation in human and rat brain following middle cerebral artery occlusion. 2007, 8:93 BMC NeurosciJournal
BMC NeuroscienceDOI
10.1186/1471-2202-8-93PubMed ID
17997827Type
ArticleLanguage
enISSN
1471-2202ae974a485f413a2113503eed53cd6c53
10.1186/1471-2202-8-93
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