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    Mrc1 and Tof1 regulate DNA replication forks in different ways during normal S phase.

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    Authors
    Hodgson, Ben
    Calzada, Arturo
    Labib, Karim
    Affiliation
    Cancer Research U.K., Paterson Institute for Cancer Research, University of Manchester, Manchester M20 4BX, United Kingdom.
    Issue Date
    2007-10
    
    Metadata
    Show full item record
    Abstract
    The Mrc1 and Tof1 proteins are conserved throughout evolution, and in budding yeast they are known to associate with the MCM helicase and regulate the progression of DNA replication forks. Previous work has shown that Mrc1 is important for the activation of checkpoint kinases in responses to defects in S phase, but both Mrc1 and Tof1 also regulate the normal process of chromosome replication. Here, we show that these two important factors control the normal progression of DNA replication forks in distinct ways. The rate of progression of DNA replication forks is greatly reduced in the absence of Mrc1 but much less affected by loss of Tof1. In contrast, Tof1 is critical for DNA replication forks to pause at diverse chromosomal sites where nonnucleosomal proteins bind very tightly to DNA, and this role is not shared with Mrc1.
    Citation
    Mrc1 and Tof1 regulate DNA replication forks in different ways during normal S phase. 2007, 18 (10):3894-902 Mol. Biol. Cell
    Journal
    Molecular Biology of the Cell
    URI
    http://hdl.handle.net/10541/70461
    DOI
    10.1091/mbc.E07-05-0500
    PubMed ID
    17652453
    Type
    Article
    Language
    en
    ISSN
    1059-1524
    ae974a485f413a2113503eed53cd6c53
    10.1091/mbc.E07-05-0500
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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