The utility of MAS5 expression summary and detection call algorithms.
Affiliation
Cancer Research UK, Paterson Institute for Cancer Research, The University of Manchester, Christie Hospital Site, Withington, Manchester, UK. spepper@picr.man.ac.uk <spepper@picr.man.ac.uk>Issue Date
2007-07-30
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BACKGROUND: Used alone, the MAS5.0 algorithm for generating expression summaries has been criticized for high False Positive rates resulting from exaggerated variance at low intensities. RESULTS: Here we show, with replicated cell line data, that, when used alongside detection calls, MAS5 can be both selective and sensitive. A set of differentially expressed transcripts were identified that were found to be changing by MAS5, but unchanging by RMA and GCRMA. Subsequent analysis by real time PCR confirmed these changes. In addition, with the Latin square datasets often used to assess expression summary algorithms, filtered MAS5.0 was found to have performance approaching that of its peers. CONCLUSION: When used alongside detection calls, MAS5 is a sensitive and selective algorithm for identifying differentially expressed genes.Citation
The utility of MAS5 expression summary and detection call algorithms. 2007, 8:273 BMC BioinformaticsJournal
BMC BioinformaticsDOI
10.1186/1471-2105-8-273PubMed ID
17663764Type
ArticleLanguage
enISSN
1471-2105ae974a485f413a2113503eed53cd6c53
10.1186/1471-2105-8-273
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