Angiogenic characteristics of circulating and tumoural thrombospondin-1 in breast cancer
dc.contributor.author | Byrne, Ged J | |
dc.contributor.author | Hayden, K | |
dc.contributor.author | McDowell, G | |
dc.contributor.author | Lang, H | |
dc.contributor.author | Kirwan, C C | |
dc.contributor.author | Tetlow, L C | |
dc.contributor.author | Kumar, Shant | |
dc.contributor.author | Bundred, Nigel J | |
dc.date.accessioned | 2009-06-12T10:39:20Z | |
dc.date.available | 2009-06-12T10:39:20Z | |
dc.date.issued | 2007-11 | |
dc.identifier.citation | Angiogenic characteristics of circulating and tumoural thrombospondin-1 in breast cancer. 2007, 31 (5):1127-32 Int. J. Oncol. | en |
dc.identifier.issn | 1019-6439 | |
dc.identifier.pmid | 17912439 | |
dc.identifier.uri | http://hdl.handle.net/10541/70266 | |
dc.description.abstract | In cancer models, thrombospondin-1 (TSP-1) has been shown to inhibit angiogenesis or promote metastasis by increasing adhesion of malignant cells to endothelium. To determine the role of TSP-1 in breast cancer and breast cancer angiogenesis, we have measured TSP-1 in plasma and tumour cytosols and compared levels to established clinicopathological prognostic parameters and intratumoural microvessel density. TSP-1 was measured, by radioimmunoassay, in plasma (pTSP-1) and tumour cytosols (cTSP-1) of women with early breast cancer (EBC) (n=71). pTSP-1 in EBC was compared to pTSP-1 levels in women with advanced breast cancer (ABC) (n=66), normal controls (n=77) and was correlated with prognostic features and microvessel density (MVD) (measured by CD31 immunostaining). cTSP-1 levels were compared to prognostic features and microvessel density. pTSP-1 in women with EBC (median 484, IQR 344-877 ng/ml) and ABC (median 588, IQR 430-952 ng/ml) were elevated when compared to normal controls (median 21, IQR 175-247) (p<0.001). Women with lymph node metastases (n=35) had higher levels of TSP-1 (median 799 ng/ml, IQR 455-943) than women who were node negative (median 343 ng/ml, IQR 267-514) (n=36) (p<0.05). Levels of pTSP-1 in EBC correlated with MVD (R=0.39, p<0.05). Levels of TSP-1 in tumour cytosols of women with EBC (median 1714, IQR 893-5283 ng/ml) correlated with microvessel density (R=0.46, p<0.01). Circulating levels of TSP-1 appear to be a marker of breast cancer aggressiveness and in breast cancer may have a pro-angiogenic rather than anti-angiogenic role. | |
dc.language.iso | en | en |
dc.subject | Breast Cancer | en |
dc.subject.mesh | Adult | |
dc.subject.mesh | Aged | |
dc.subject.mesh | Aged, 80 and over | |
dc.subject.mesh | Breast Neoplasms | |
dc.subject.mesh | Cytosol | |
dc.subject.mesh | Female | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Middle Aged | |
dc.subject.mesh | Neovascularization, Pathologic | |
dc.subject.mesh | Thrombospondin 1 | |
dc.title | Angiogenic characteristics of circulating and tumoural thrombospondin-1 in breast cancer | en |
dc.type | Article | en |
dc.contributor.department | Department of Surgery, South Manchester University Hospitals, Wythenshawe Hospital, Manchester, UK. | en |
dc.identifier.journal | International Journal of Oncology | en |
html.description.abstract | In cancer models, thrombospondin-1 (TSP-1) has been shown to inhibit angiogenesis or promote metastasis by increasing adhesion of malignant cells to endothelium. To determine the role of TSP-1 in breast cancer and breast cancer angiogenesis, we have measured TSP-1 in plasma and tumour cytosols and compared levels to established clinicopathological prognostic parameters and intratumoural microvessel density. TSP-1 was measured, by radioimmunoassay, in plasma (pTSP-1) and tumour cytosols (cTSP-1) of women with early breast cancer (EBC) (n=71). pTSP-1 in EBC was compared to pTSP-1 levels in women with advanced breast cancer (ABC) (n=66), normal controls (n=77) and was correlated with prognostic features and microvessel density (MVD) (measured by CD31 immunostaining). cTSP-1 levels were compared to prognostic features and microvessel density. pTSP-1 in women with EBC (median 484, IQR 344-877 ng/ml) and ABC (median 588, IQR 430-952 ng/ml) were elevated when compared to normal controls (median 21, IQR 175-247) (p<0.001). Women with lymph node metastases (n=35) had higher levels of TSP-1 (median 799 ng/ml, IQR 455-943) than women who were node negative (median 343 ng/ml, IQR 267-514) (n=36) (p<0.05). Levels of pTSP-1 in EBC correlated with MVD (R=0.39, p<0.05). Levels of TSP-1 in tumour cytosols of women with EBC (median 1714, IQR 893-5283 ng/ml) correlated with microvessel density (R=0.46, p<0.01). Circulating levels of TSP-1 appear to be a marker of breast cancer aggressiveness and in breast cancer may have a pro-angiogenic rather than anti-angiogenic role. |