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    Investigation of downstream target genes of PAX3c, PAX3e and PAX3g isoforms in melanocytes by microarray analysis.

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    Authors
    Wang, Qiuyu
    Kumar, Shant
    Mitsios, Nick
    Slevin, Mark
    Kumar, Patricia
    Affiliation
    School of Biology, Chemistry and Health Science, Manchester Metropolitan University, Manchester, United Kingdom.
    Issue Date
    2007-03-15
    
    Metadata
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    Abstract
    PAX3 encodes a transcription factor, which with Zic1 is necessary for induction of the neural crest during early embryonic development. There are 7 human PAX3 isoforms (a-h). PAX3e is the full length isoform comprising 10 exons. PAX3c comprises 8 exons plus 5 codons of intron 8, while PAX3g has a truncated transactivation domain. Previous studies by us indicated that these isoforms have different activities in melanocytes in vitro. In this study, a mouse gene oligo array ( approximately 7.5 k oligos), from the Human Genome Mapping Project (HGMP) Resource Centre, was used to screen for alterations in downstream gene expression in PAX3c, PAX3e and PAX3g melanocyte transfectants, compared with empty vector controls. The data analyses identified 109 genes up or downregulated, at least 2-fold, and involved in cell differentiation, proliferation, migration, adhesion, apoptosis and angiogenesis. Semi-quantitative RT-PCR and Western blotting confirmed the changes identified by microarrays for several putative targets of PAX3, including Met, MyoD and Muc18, and previously undescribed targets, including Dhh, Fgf17, Kitl and Rac1. Thus, our data reveal that PAX3 isoforms regulate distinct but overlapping sets of genes in melanocytes in vitro.
    Citation
    Investigation of downstream target genes of PAX3c, PAX3e and PAX3g isoforms in melanocytes by microarray analysis. 2007, 120 (6):1223-31 Int. J. Cancer
    Journal
    International Journal of Cancer.
    URI
    http://hdl.handle.net/10541/70158
    DOI
    10.1002/ijc.22316
    PubMed ID
    17187370
    Type
    Article
    Language
    en
    ISSN
    0020-7136
    ae974a485f413a2113503eed53cd6c53
    10.1002/ijc.22316
    Scopus Count
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