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dc.contributor.authorNguyen Huu, Ngoc Sa
dc.contributor.authorRyder, W David J
dc.contributor.authorZeps, N
dc.contributor.authorFlasza, Marzena
dc.contributor.authorChiu, M
dc.contributor.authorHanby, A M
dc.contributor.authorPoulsom, R
dc.contributor.authorClarke, Robert B
dc.contributor.authorBaron, M
dc.date.accessioned2009-05-21T16:40:57Z
dc.date.available2009-05-21T16:40:57Z
dc.date.issued2008-09
dc.identifier.citationTumour-promoting activity of altered WWP1 expression in breast cancer and its utility as a prognostic indicator. 2008, 216 (1):93-102 J. Pathol.en
dc.identifier.issn0022-3417
dc.identifier.pmid18604872
dc.identifier.doi10.1002/path.2385
dc.identifier.urihttp://hdl.handle.net/10541/68740
dc.description.abstractWWP1 is a ubiquitin ligase, associated with the post-translational regulation of several tumour-promoting and tumour suppressor proteins. Here we show that WWP1 expression is up-regulated in a subset of breast tumour cell lines and primary breast tumours. We overexpressed WWP1 in MCF10A breast epithelial cells and demonstrated increased cell growth and anchorage-independent colony formation. RNAi knockdown of WWP1 expression in T47D and MCF7 breast tumour cell lines reduced anchorage-independent colony formation. We used WWP1 protein expression levels, in combination with its sub-cellular localization, to classify breast tumours into four categories. Surprisingly, a category with low/absent WWP1 expression displayed a consistently worse prognosis compared with WWP1-expressing tumours. Importantly, the association with disease-free survival was independent of the status of other commonly used prognostic indicators. Thus, WWP1 is a prognostic marker and may be a potential therapeutic target for a subset of breast tumours.
dc.language.isoenen
dc.subjectBreast Canceren
dc.subjectTumour Markersen
dc.subjectNedd4 Familyen
dc.subjectWWP1en
dc.subject.meshAdolescent
dc.subject.meshAdult
dc.subject.meshAged
dc.subject.meshAged, 80 and over
dc.subject.meshBreast Neoplasms
dc.subject.meshGene Expression Regulation, Neoplastic
dc.subject.meshHumans
dc.subject.meshMiddle Aged
dc.subject.meshOncogenes
dc.subject.meshPrognosis
dc.subject.meshRegression Analysis
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
dc.subject.meshTumor Markers, Biological
dc.subject.meshUbiquitin-Protein Ligases
dc.titleTumour-promoting activity of altered WWP1 expression in breast cancer and its utility as a prognostic indicator.en
dc.typeArticleen
dc.contributor.departmentUniversity of Manchester, Faculty of Life Sciences, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK.en
dc.identifier.journalThe Journal of Pathologyen
html.description.abstractWWP1 is a ubiquitin ligase, associated with the post-translational regulation of several tumour-promoting and tumour suppressor proteins. Here we show that WWP1 expression is up-regulated in a subset of breast tumour cell lines and primary breast tumours. We overexpressed WWP1 in MCF10A breast epithelial cells and demonstrated increased cell growth and anchorage-independent colony formation. RNAi knockdown of WWP1 expression in T47D and MCF7 breast tumour cell lines reduced anchorage-independent colony formation. We used WWP1 protein expression levels, in combination with its sub-cellular localization, to classify breast tumours into four categories. Surprisingly, a category with low/absent WWP1 expression displayed a consistently worse prognosis compared with WWP1-expressing tumours. Importantly, the association with disease-free survival was independent of the status of other commonly used prognostic indicators. Thus, WWP1 is a prognostic marker and may be a potential therapeutic target for a subset of breast tumours.


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