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    NEP-A and NEP-B both contribute to nuclear pore formation in Xenopus eggs and oocytes.

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    Authors
    Salpingidou, Georgia
    Rzepecki, Ryszard
    Kiseleva, Elena
    Lyon, Carol
    Lane, Birgit
    Fusiek, Kasia
    Golebiewska, Anja
    Drummond, Sheona P
    Allen, Terence D
    Ellis, Juliet A
    Smythe, Carl
    Goldberg, Martin W
    Hutchison, Christopher J
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    Affiliation
    Integrative Cell Biology Laboratories, School of Biological and Biomedical Sciences, The University of Durham, South Road, Durham DH1 3LE, UK.
    Issue Date
    2008-03-01
    
    Metadata
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    Abstract
    In vertebrates, the nuclear envelope (NE) assembles and disassembles during mitosis. As the NE is a complex structure consisting of inner and outer membranes, nuclear pore complexes (NPCs) and the nuclear lamina, NE assembly must be a controlled and systematic process. In Xenopus egg extracts, NE assembly is mediated by two distinct membrane vesicle populations, termed NEP-A and NEP-B. Here, we re-investigate how these two membrane populations contribute to NPC assembly. In growing stage III Xenopus oocytes, NPC assembly intermediates are frequently observed. High concentrations of NPC assembly intermediates always correlate with fusion of vesicles into preformed membranes. In Xenopus egg extracts, two integral membrane proteins essential for NPC assembly, POM121 and NDC1, are exclusively associated with NEP-B membranes. By contrast, a third integral membrane protein associated with the NPCs, gp210, associates only with NEP-A membranes. During NE assembly, fusion between NEP-A and NEP-B led to the formation of fusion junctions at which >65% of assembling NPCs were located. To investigate how each membrane type contributes to NPC assembly, we preferentially limited NEP-A in NE assembly assays. We found that, by limiting the NEP-A contribution to the NE, partially formed NPCs were assembled in which protein components of the nucleoplasmic face were depleted or absent. Our data suggest that fusion between NEP-A and NEP-B membranes is essential for NPC assembly and that, in contrast to previous reports, both membranes contribute to NPC assembly.
    Citation
    NEP-A and NEP-B both contribute to nuclear pore formation in Xenopus eggs and oocytes. 2008, 121 (Pt 5):706-16 J. Cell. Sci.
    Journal
    Journal of Cell Science
    URI
    http://hdl.handle.net/10541/68739
    DOI
    10.1242/jcs.019968
    PubMed ID
    18270266
    Type
    Article
    Language
    en
    ISSN
    0021-9533
    ae974a485f413a2113503eed53cd6c53
    10.1242/jcs.019968
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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