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    A casein kinase 1 and PAR proteins regulate asymmetry of a PIP(2) synthesis enzyme for asymmetric spindle positioning.

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    Authors
    Panbianco, Costanza
    Weinkove, David
    Zanin, Esther
    Jones, David R
    Divecha, Nullin
    Gotta, Monica
    Ahringer, Julie
    Affiliation
    The Gurdon Institute and Department of Genetics, University of Cambridge, Tennis Court Road, Cambridge CB21QN, UK.
    Issue Date
    2008-08
    
    Metadata
    Show full item record
    Abstract
    Spindle positioning is an essential feature of asymmetric cell division. The conserved PAR proteins together with heterotrimeric G proteins control spindle positioning in animal cells, but how these are linked is not known. In C. elegans, PAR protein activity leads to asymmetric spindle placement through cortical asymmetry of Galpha regulators GPR-1/2. Here, we establish that the casein kinase 1 gamma CSNK-1 and a PIP(2) synthesis enzyme (PPK-1) transduce PAR polarity to asymmetric Galpha regulation. PPK-1 is posteriorly enriched in the one-celled embryo through PAR and CSNK-1 activities. Loss of CSNK-1 causes uniformly high PPK-1 levels, high symmetric cortical levels of GPR-1/2 and LIN-5, and increased spindle pulling forces. In contrast, knockdown of ppk-1 leads to low GPR-1/2 levels and decreased spindle forces. Furthermore, loss of CSNK-1 leads to increased levels of PIP(2). We propose that asymmetric generation of PIP(2) by PPK-1 directs the posterior enrichment of GPR-1/2 and LIN-5, leading to posterior spindle displacement.
    Citation
    A casein kinase 1 and PAR proteins regulate asymmetry of a PIP(2) synthesis enzyme for asymmetric spindle positioning. 2008, 15 (2):198-208 Dev. Cell
    Journal
    Developmental Cell
    URI
    http://hdl.handle.net/10541/68736
    DOI
    10.1016/j.devcel.2008.06.002
    PubMed ID
    18694560
    Type
    Article
    Language
    en
    ISSN
    1534-5807
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.devcel.2008.06.002
    Scopus Count
    Collections
    Inositide Laboratory
    All Paterson Institute for Cancer Research

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