Leukaemia inhibitory factor is over-expressed by ischaemic brain tissue concomitant with reduced plasma expression following acute stroke.
AffiliationThe Department of Biology, Chemistry and Health Science, Manchester Metropolitan University, Manchester, UK. email@example.com
MetadataShow full item record
AbstractLeukaemia inhibitory factor (LIF) is a glycoprotein of the interleukin-6 family, which has potent pro-inflammatory properties and is involved in regulation of neuronal differentiation. We have previously identified its upregulation in gene microarrays following acute ischaemic stroke in man. LIF expression and localization was measured in human ischaemic stroke autopsy specimens, in a rat model of middle cerebral artery occlusion (MCAO) and in human foetal neural cell cultures following oxygen-glucose deprivation (OGD) by Western blotting and immunohistochemistry. Circulating LIF was determined in the plasma of patients in the hyper-acute stroke phase using a multiplex enzyme-linked-immunosorbent serologic assay system. Patients demonstrated an increase in LIF expression in peri-infarcted regions with localization in neurons and endothelial cells of microvessels surrounding the infarcted core. The rat MCAO model showed similar upregulation in neurons with a peak increase at 90 min. Circulating serum LIF expression was significantly decreased in the hyper-acute phase of stroke. Brain-derived neurons and glia cultured in vitro demonstrated an increase in gene/protein and protein expression respectively following exposure to OGD. Increased LIF expression in peri-infarcted regions and sequestration from the peripheral circulation in acute stroke patients are characteristic of the pathobiological response to ischaemia and tissue damage.
CitationLeukaemia inhibitory factor is over-expressed by ischaemic brain tissue concomitant with reduced plasma expression following acute stroke. 2008, 15 (1):29-37 Eur. J. Neurol.
JournalEuropean Journal of Neurology
- Cellular prion protein is increased in the plasma and peri-infarcted brain tissue after acute stroke.
- Authors: Mitsios N, Saka M, Krupinski J, Pennucci R, Sanfeliu C, Miguel Turu M, Gaffney J, Kumar P, Kumar S, Sullivan M, Slevin M
- Issue date: 2007 Feb 15
- Peroxisome proliferator-activated receptorsgamma (PPARgamma) differently modulate the interleukin-6 expression in the peri-infarct cortical tissue in the acute and delayed phases of cerebral ischaemia.
- Authors: Patzer A, Zhao Y, Stöck I, Gohlke P, Herdegen T, Culman J
- Issue date: 2008 Nov
- Neuroprotective activity of leukemia inhibitory factor is relayed through myeloid zinc finger-1 in a rat model of stroke.
- Authors: Davis SM, Collier LA, Foran EA, Leonardo CC, Ajmo CT Jr, Pennypacker KR
- Issue date: 2019 Apr
- Changes in hyaluronan production and metabolism following ischaemic stroke in man.
- Authors: Al'Qteishat A, Gaffney J, Krupinski J, Rubio F, West D, Kumar S, Kumar P, Mitsios N, Slevin M
- Issue date: 2006 Aug
- Impact of microRNA-134 on neural cell survival against ischemic injury in primary cultured neuronal cells and mouse brain with ischemic stroke by targeting HSPA12B.
- Authors: Chi W, Meng F, Li Y, Li P, Wang G, Cheng H, Han S, Li J
- Issue date: 2014 Dec 10