AuthorsAllen, Terence D
Rutherford, Sandra A
Murray, Stephen M
Drummond, Sheona P
Goldberg, Martin W
AffiliationDepartment of Structural Cell Biology, Paterson Institute for Cancer Research, University of Manchester, Manchester M20 4BX, United Kingdom.
MetadataShow full item record
AbstractAccessing internal structure and retaining relative three dimensional (3D) organization within the nucleus has always proved difficult in the electron microscope. This is due to the overall size and largely fibrous nature of the contents, making large scale 3D reconstructions difficult from thin sections using transmission electron microscopy. This chapter brings together a number of methods developed for visualization of nuclear structure by scanning electron microscopy (SEM). These methods utilize the easily accessed high resolution available in field emission instruments. Surface imaging has proved particularly useful to date in studies of the nuclear envelope and pore complexes, and has also shown promise for internal nuclear organization, including the dynamic and radical reorganization of structure during cell division. Consequently, surface imaging in the SEM has the potential to make a significant contribution to our understanding of nuclear structure.
CitationScanning electron microscopy of nuclear structure. 2008, 88:389-409 Methods Cell Biol.
JournalMethods in Cell Biology
- Accessing nuclear structure for field emission, in lens, scanning electron microscopy (FEISEM).
- Authors: Allen TD, Bennion GR, Rutherford SA, Reipert S, Ramalho A, Kiseleva E, Goldberg MW
- Issue date: 1996
- Oocytes as an experimental system to analyze the ultrastructure of endogenous and ectopically expressed nuclear envelope components by field-emission scanning electron microscopy.
- Authors: Stick R, Goldberg MW
- Issue date: 2010 May
- Visualization of the nucleus and nuclear envelope in situ by SEM in tissue culture cells.
- Authors: Allen TD, Rutherford SA, Murray S, Gardiner F, Kiseleva E, Goldberg MW, Drummond SP
- Issue date: 2007
- A protocol for isolating Xenopus oocyte nuclear envelope for visualization and characterization by scanning electron microscopy (SEM) or transmission electron microscopy (TEM).
- Authors: Allen TD, Rutherford SA, Murray S, Sanderson HS, Gardiner F, Kiseleva E, Goldberg MW, Drummond SP
- Issue date: 2007
- The nuclear pore complex: three-dimensional surface structure revealed by field emission, in-lens scanning electron microscopy, with underlying structure uncovered by proteolysis.
- Authors: Goldberg MW, Allen TD
- Issue date: 1993 Sep