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dc.contributor.authorSweeney, Elizabeth
dc.contributor.authorWard, Timothy H
dc.contributor.authorGray, N
dc.contributor.authorWomack, C
dc.contributor.authorJayson, Gordon C
dc.contributor.authorHughes, Andrew
dc.contributor.authorDive, Caroline
dc.contributor.authorByers, Richard J
dc.date.accessioned2009-05-12T18:38:28Z
dc.date.available2009-05-12T18:38:28Z
dc.date.issued2008-09-19
dc.identifier.citationQuantitative multiplexed quantum dot immunohistochemistry. 2008, 374 (2):181-6 Biochem. Biophys. Res. Commun.en
dc.identifier.issn1090-2104
dc.identifier.pmid18621021
dc.identifier.doi10.1016/j.bbrc.2008.06.127
dc.identifier.urihttp://hdl.handle.net/10541/67954
dc.description.abstractQuantum dots are photostable fluorescent semiconductor nanocrystals possessing wide excitation and bright narrow, symmetrical, emission spectra. These characteristics have engendered considerable interest in their application in multiplex immunohistochemistry for biomarker quantification and co-localisation in clinical samples. Robust quantitation allows biomarker validation, and there is growing need for multiplex staining due to limited quantity of clinical samples. Most reported multiplexed quantum dot staining used sequential methods that are laborious and impractical in a high-throughput setting. Problems associated with sequential multiplex staining have been investigated and a method developed using QDs conjugated to biotinylated primary antibodies, enabling simultaneous multiplex staining with three antibodies. CD34, Cytokeratin 18 and cleaved Caspase 3 were triplexed in tonsillar tissue using an 8h protocol, each localised to separate cellular compartments. This demonstrates utility of the method for biomarker measurement enabling rapid measurement of multiple co-localised biomarkers on single paraffin tissue sections, of importance for clinical trial studies.
dc.language.isoenen
dc.subjectQDoten
dc.subjectNanocrystalen
dc.subjectSpectral Imagingen
dc.subject.meshAntibodies
dc.subject.meshAntigens, CD34
dc.subject.meshBiotinylation
dc.subject.meshCaspase 3
dc.subject.meshCells, Cultured
dc.subject.meshHumans
dc.subject.meshImmunohistochemistry
dc.subject.meshKeratin-18
dc.subject.meshPalatine Tonsil
dc.subject.meshQuantum Dots
dc.titleQuantitative multiplexed quantum dot immunohistochemistry.en
dc.typeArticleen
dc.contributor.departmentClinical and Experimental Pharmacology, Paterson Institute for Cancer Research, Wilmslow Road, Manchester, 420 4BX, UK.en
dc.identifier.journalBiochemical and Biophysical Research Communicationsen
html.description.abstractQuantum dots are photostable fluorescent semiconductor nanocrystals possessing wide excitation and bright narrow, symmetrical, emission spectra. These characteristics have engendered considerable interest in their application in multiplex immunohistochemistry for biomarker quantification and co-localisation in clinical samples. Robust quantitation allows biomarker validation, and there is growing need for multiplex staining due to limited quantity of clinical samples. Most reported multiplexed quantum dot staining used sequential methods that are laborious and impractical in a high-throughput setting. Problems associated with sequential multiplex staining have been investigated and a method developed using QDs conjugated to biotinylated primary antibodies, enabling simultaneous multiplex staining with three antibodies. CD34, Cytokeratin 18 and cleaved Caspase 3 were triplexed in tonsillar tissue using an 8h protocol, each localised to separate cellular compartments. This demonstrates utility of the method for biomarker measurement enabling rapid measurement of multiple co-localised biomarkers on single paraffin tissue sections, of importance for clinical trial studies.


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