FTIR-based spectroscopic analysis in the identification of clinically aggressive prostate cancer.
AffiliationManchester Interdisciplinary Biocentre, Centre for Instrumentation and Analytical Science, School of Chemical Engineering and Analytical Science, The University of Manchester, Manchester, M1 7DN, UK. M.J.Baker@manchester.ac.uk
MetadataShow full item record
AbstractFourier transform infrared (FTIR) spectroscopy is a vibrational spectroscopic technique that uses infrared radiation to vibrate molecular bonds within the sample that absorbs it. As different samples contain different molecular bonds or different configurations of molecular bonds, FTIR allows us to obtain chemical information on molecules within the sample. Fourier transform infrared microspectroscopy in conjunction with a principal component-discriminant function analysis (PC-DFA) algorithm was applied to the grading of prostate cancer (CaP) tissue specimens. The PC-DFA algorithm is used alongside the established diagnostic measures of Gleason grading and the tumour/node/metastasis system. Principal component-discriminant function analysis improved the sensitivity and specificity of a three-band Gleason score criterion diagnosis previously reported by attaining an overall sensitivity of 92.3% and specificity of 99.4%. For the first time, we present the use of a two-band criterion showing an association of FTIR-based spectral characteristics with clinically aggressive behaviour in CaP manifest as local and/or distal spread. This paper shows the potential for the use of spectroscopic analysis for the evaluation of the biopotential of CaP in an accurate and reproducible manner.
CitationFTIR-based spectroscopic analysis in the identification of clinically aggressive prostate cancer. 2008, 99 (11):1859-66 Br. J. Cancer
JournalBritish Journal of Cancer
- Investigating FTIR based histopathology for the diagnosis of prostate cancer.
- Authors: Baker MJ, Gazi E, Brown MD, Shanks JH, Clarke NW, Gardner P
- Issue date: 2009 Feb
- A correlation of FTIR spectra derived from prostate cancer biopsies with gleason grade and tumour stage.
- Authors: Gazi E, Baker M, Dwyer J, Lockyer NP, Gardner P, Shanks JH, Reeve RS, Hart CA, Clarke NW, Brown MD
- Issue date: 2006 Oct
- The combined application of FTIR microspectroscopy and ToF-SIMS imaging in the study of prostate cancer.
- Authors: Gazi E, Dwyer J, Lockyer N, Gardner P, Vickerman JC, Miyan J, Hart CA, Brown M, Shanks JH, Clarke N
- Issue date: 2004
- Applications of Fourier transform infrared microspectroscopy in studies of benign prostate and prostate cancer. A pilot study.
- Authors: Gazi E, Dwyer J, Gardner P, Ghanbari-Siahkali A, Wade AP, Miyan J, Lockyer NP, Vickerman JC, Clarke NW, Shanks JH, Scott LJ, Hart CA, Brown M
- Issue date: 2003 Sep
- Characterization of normal and malignant prostate tissue by Fourier transform infrared microspectroscopy.
- Authors: Pezzei C, Pallua JD, Schaefer G, Seifarth C, Huck-Pezzei V, Bittner LK, Klocker H, Bartsch G, Bonn GK, Huck CW
- Issue date: 2010 Nov