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    Characterization of the Hoechst 33342 side population from normal and malignant human renal epithelial cells.

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    Authors
    Addla, Sanjai K
    Brown, Michael D
    Hart, Claire A
    Ramani, Vijay A C
    Clarke, Noel W
    Affiliation
    Genito-Urinary Cancer Research Group, School of Cancer and Imaging Sciences, Paterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Manchester, UK.
    Issue Date
    2008-09
    
    Metadata
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    Abstract
    The fundamental changes which predispose for renal cell carcinoma (RCC) are poorly characterized. It is hypothesized that "cancer stem cells" may be influential in carcinogenesis, and the epithelial side population (SP) is enriched for stemlike cells in other epithelial cancers. In this study, we have isolated and characterized the SP and non-SP (NSP) populations from normal (NK) and malignant (RCC) human kidney tissue. NK specimens were taken from patients undergoing non-renal cancer surgery and paired malignant and macroscopically normal tissue samples were taken from patients undergoing surgery for RCC. The Hoechst 33342 dye efflux technique was used to isolate epithelial SP and NSP from normal and malignant human renal tissue. Cellular subpopulations were phenotyped for lineage, cell cycle, and putative stem cell markers, and functionally characterized using in vitro colony-forming and proliferation assays. The SP constituted 3.8 +/- 0.4 and 5.9 +/- 0.9% of epithelial cells in NK and RCC, respectively, of which 14.1 +/- 3.5 and 13.2 +/- 3.6% were shown to be in G(0). SP cells demonstrated greater proliferative potential in colony-forming efficiency, long-term culture, and spheroids assays and were shown to be maintained upon tissue culture passage. We have shown that the renal SP is enriched for quiescent cells, with a high proliferative capacity and stemlike properties. The population is, however, heterogeneous, confirming that the terms "SP cell" and "stem cell" cannot be used interchangeably.
    Citation
    Characterization of the Hoechst 33342 side population from normal and malignant human renal epithelial cells. 2008, 295 (3):F680-7 Am. J. Physiol. Renal Physiol.
    Journal
    American journal of Physiology. Renal Physiology
    URI
    http://hdl.handle.net/10541/67947
    DOI
    10.1152/ajprenal.90286.2008
    PubMed ID
    18614618
    Type
    Article
    Language
    en
    ISSN
    0363-6127
    ae974a485f413a2113503eed53cd6c53
    10.1152/ajprenal.90286.2008
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research
    Urological Oncology
    School of Cancer and Imaging Sciences

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