Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast.
Authors
Sanchez-Diaz, AlbertoMarchesi, Vanessa
Murray, Stephen M
Jones, Richard C
Pereira, Gislene
Edmondson, Ricky D
Allen, Terence D
Labib, Karim
Affiliation
Cancer Research U.K., Paterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Manchester, M20 4BX, UK.Issue Date
2008-04
Metadata
Show full item recordAbstract
By rapidly depleting each of the essential budding yeast proteins of unknown function, we identified a novel factor that we call Inn1, which associates with the contractile actomyosin ring at the end of mitosis and is needed for cytokinesis. We show that Inn1 has a C2 domain at the amino terminus of the protein that is required for ingression of the plasma membrane, whereas the remainder of the protein recruits Inn1 to the actomyosin ring. The lethal effects of deleting the INN1 gene can be suppressed by artificial fusion of the C2 domain to other components of the actomyosin ring, restoring membrane ingression on contraction of the actomyosin ring. Our data indicate that recruitment of the C2 domain of Inn1 to the contractile actomyosin ring is crucial for ingression of the plasma membrane during cytokinesis in budding yeast.Citation
Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast. 2008, 10 (4):395-406 Nat. Cell Biol.Journal
Nature Cell BiologyDOI
10.1038/ncb1701PubMed ID
18344988Type
ArticleLanguage
enISSN
1476-4679ae974a485f413a2113503eed53cd6c53
10.1038/ncb1701
Scopus Count
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