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dc.contributor.authorPolenkowski, M.
dc.contributor.authorAllister, A. B.
dc.contributor.authorBurbano de Lara, S.
dc.contributor.authorPierce, A.
dc.contributor.authorGeary, Bethany
dc.contributor.authorEl Bounkari, O.
dc.contributor.authorWiehlmann, L.
dc.contributor.authorHoffmann, A.
dc.contributor.authorWhetton, A. D.
dc.contributor.authorTamura, T.
dc.contributor.authorTran, D. D. H.
dc.date.accessioned2023-01-24T10:32:17Z
dc.date.available2023-01-24T10:32:17Z
dc.date.issued2023en
dc.identifier.citationPolenkowski M, Allister AB, Burbano de Lara S, Pierce A, Geary B, El Bounkari O, et al. THOC5 complexes with DDX5, DDX17, and CDK12 to regulate R loop structures and transcription elongation rate. iScience. 2023 Jan 20;26(1):105784. PubMed PMID: 36590164. Pubmed Central PMCID: PMC9800341. Epub 2023/01/03. eng.en
dc.identifier.pmid36590164en
dc.identifier.doi10.1016/j.isci.2022.105784en
dc.identifier.urihttp://hdl.handle.net/10541/625936
dc.description.abstractTHOC5, a member of the THO complex, is essential for the 3'processing of some inducible genes, the export of a subset of mRNAs and stem cell survival. Here we show that THOC5 depletion results in altered 3'cleavage of >50% of mRNAs and changes in RNA polymerase II binding across genes. THOC5 is recruited close to high-density polymerase II sites, suggesting that THOC5 is involved in transcriptional elongation. Indeed, measurement of elongation rates in vivo demonstrated decreased rates in THOC5-depleted cells. Furthermore, THOC5 is preferentially recruited to its target genes in slow polymerase II cells compared with fast polymerase II cells. Importantly chromatin-associated THOC5 interacts with CDK12 (a modulator of transcription elongation) and RNA helicases DDX5, DDX17, and THOC6 only in slow polymerase II cells. The CDK12/THOC5 interaction promotes CDK12 recruitment to R-loops in a THOC6-dependent manner. These data demonstrate a novel function of THOC5 in transcription elongation.en
dc.language.isoenen
dc.relation.urlhttps://dx.doi.org/10.1016/j.isci.2022.105784en
dc.titleTHOC5 complexes with DDX5, DDX17, and CDK12 to regulate R loop structures and transcription elongation rateen
dc.typeArticleen
dc.contributor.departmentDepartment of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Hannover D-30623, Germanyen
dc.identifier.journaliScienceen
dc.description.noteen]
refterms.dateFOA2023-01-24T13:29:00Z


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