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dc.contributor.authorKathirvel, K.
dc.contributor.authorKaren, L.
dc.contributor.authorHaribalaganesh, R.
dc.contributor.authorKrishnadas, R.
dc.contributor.authorMuthukkaruppan, V.
dc.contributor.authorLane, Brian
dc.contributor.authorSimpson, D. A.
dc.contributor.authorGoljanek-Whysall, K.
dc.contributor.authorSheridan, C.
dc.contributor.authorBharanidharan, D.
dc.contributor.authorWilloughby, C. E.
dc.contributor.authorSenthilkumari, S.
dc.date.accessioned2022-06-22T07:18:38Z
dc.date.available2022-06-22T07:18:38Z
dc.date.issued2022en
dc.identifier.citationKathirvel K, Karen L, Haribalaganesh R, Krishnadas R, Muthukkaruppan V, Lane B, et al. Short and long-term effect of dexamethasone on the transcriptome profile of primary human trabecular meshwork cells in vitro. Vol. 12, Scientific Reports. Springer Science and Business Media LLC; 2022. en
dc.identifier.pmid35585182en
dc.identifier.doi10.1038/s41598-022-12443-7en
dc.identifier.urihttp://hdl.handle.net/10541/625347
dc.description.abstractIn the quest of identifying newer molecular targets for the management of glucocorticoid-induced ocular hypertension (GC-OHT) and glaucoma (GCG), several microarray studies have attempted to investigate the genome-wide transcriptome profiling of primary human trabecular meshwork (TM) cells in response to dexamethasone (DEX). However, no studies are reported so far to demonstrate the temporal changes in the expression of genes in the cultured human TM cells in response to DEX treatment. Therefore, in the present study, the time-dependent changes in the genome-wide expression of genes in primary human TM cells after short (16 hours: 16 h) and long exposure (7 days: 7 d) of DEX was investigated using RNA sequencing. There were 199 (118 up-regulated; 81 down-regulated) and 525 (119 up-regulated; 406 down-regulated) DEGs in 16 h and 7 d treatment groups respectively. The unique genes identified in 16 h and 7 d treatment groups were 152 and 478 respectively. This study found a distinct gene signature and pathways between two treatment regimes. Longer exposure of DEX treatment showed a dys-regulation of Wnt and Rap1 signaling and so highlighted potential therapeutic targets for pharmacological management of GC-OHT/glaucoen
dc.language.isoenen
dc.relation.urlhttps://dx.doi.org/10.1038/s41598-022-12443-7en
dc.titleShort and long-term effect of dexamethasone on the transcriptome profile of primary human trabecular meshwork cells in vitroen
dc.typeArticleen
dc.contributor.departmentDepartment of Ocular Pharmacology, Aravind Medical Research Foundation, #1, Anna Nagar, Madurai, Tamil Nadu, 625020, Indiaen
dc.identifier.journalScientific Reportsen
dc.description.noteen]
refterms.dateFOA2022-06-22T12:20:54Z


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