• Login
    View Item 
    •   Home
    • The Christie Research Publications Repository
    • All Christie Publications
    • View Item
    •   Home
    • The Christie Research Publications Repository
    • All Christie Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Apoptotic priming is defined by the dynamic exchange of Bcl-2 proteins between mitochondria and cytosol

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    35585181.pdf
    Size:
    5.442Mb
    Format:
    PDF
    Download
    Authors
    King, L. E.
    Rodriguez-Enriquez, R.
    Pedley, R.
    Mellor, C. E. L.
    Wang, Pengbo
    Zindy, E.
    White, M. R. H.
    Brennan, K.
    Gilmore, Andrew P
    Affiliation
    Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, Manchester, UK
    Issue Date
    2022
    
    Metadata
    Show full item record
    Abstract
    Apoptosis is regulated by interactions between the BH3-only and multi-domain Bcl-2 family proteins. These interactions are integrated on the outer mitochondrial membrane (OMM) where they set the threshold for apoptosis, known as mitochondrial priming. However, how mitochondrial priming is controlled at the level of single cells remains unclear. Retrotranslocation of Bcl-XL has been proposed as one mechanism, removing pro-apoptotic Bcl-2 proteins from the OMM, thus reducing priming. Contrary to this view, we now show that Bcl-XL retrotranslocation is inhibited by binding to its BH3-only partners, resulting in accumulation of these protein complexes on mitochondria. We find that Bcl-XL retrotranslocation dynamics are tightly coupled to mitochondrial priming. Quantifying these dynamics indicates the heterogeneity in priming between cells within a population and predicts how they subsequently respond to a pro-apoptotic signal.
    Citation
    King LE, Rodriguez-Enriquez R, Pedley R, Mellor CEL, Wang P, Zindy E, et al. Apoptotic priming is defined by the dynamic exchange of Bcl-2 proteins between mitochondria and cytosol. Cell Death & Differentiation. Springer Science and Business Media LLC; 2022.
    Journal
    Cell Death and Differentiation
    URI
    http://hdl.handle.net/10541/625320
    DOI
    10.1038/s41418-022-01013-z
    PubMed ID
    35585181
    Additional Links
    https://dx.doi.org/10.1038/s41418-022-01013-z
    Type
    Article
    Language
    en
    ae974a485f413a2113503eed53cd6c53
    10.1038/s41418-022-01013-z
    Scopus Count
    Collections
    All Christie Publications

    entitlement

    Related articles

    • Assessment of Dynamic BCL-2 Protein Shuttling Between Outer Mitochondrial Membrane and Cytosol.
    • Authors: Lauterwasser J, Fimm-Todt F, Edlich F
    • Issue date: 2019
    • Pro-apoptotic complexes of BAX and BAK on the outer mitochondrial membrane.
    • Authors: Wolf P, Schoeniger A, Edlich F
    • Issue date: 2022 Oct
    • Bad is essential for Bcl-xL-enhanced Bax shuttling between mitochondria and cytosol.
    • Authors: Mai Z, Sun H, Yang F, Du M, Cheng X, Chen H, Sun B, Wen J, Wang X, Chen T
    • Issue date: 2023 Feb
    • BimS-induced apoptosis requires mitochondrial localization but not interaction with anti-apoptotic Bcl-2 proteins.
    • Authors: Weber A, Paschen SA, Heger K, Wilfling F, Frankenberg T, Bauerschmitt H, Seiffert BM, Kirschnek S, Wagner H, Häcker G
    • Issue date: 2007 May 21
    • BCL-2 proteins and apoptosis: Recent insights and unknowns.
    • Authors: Edlich F
    • Issue date: 2018 May 27
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.