A novel 5x multiplex immunohistochemical staining reveals PSMA as a helpful marker in prostate cancer with low p504s expression
AuthorsRüschoff, J. H.
Fankhauser, Christian D
Wild, P. J.
Rupp, N. J.
AffiliationDepartment of Pathology and Molecular Pathology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
MetadataShow full item record
AbstractThe ability to combine multiple immunohistochemical (IHC) markers within a single tissue section facilitates the evaluation and detection of co-expressions, while saving tissue. A newly developed 5x multiplex (MPX) IHC staining of five different IHC markers (Basal cell cocktail (34βE12 + p63), p504s (SP116), ERG (EPR3864), Ki-67 (30-9), PSMA (EP192)) was applied on whole sections of n = 37 radical prostatectomies (RPE) including normal and cancerous tissue. Four different colors including brown, magenta, yellow and teal coded for different stainings, whereas magenta was used twice for nuclear Ki-67 and cytosolic / membranous PSMA. The staining of multiplex IHC was compared to single stains of ERG, PSMA and p504s. The proper staining of the basal cell cocktail and Ki-67 could be assessed by internal positive controls in the multiplex staining. The proportion of PSMA and p504s expression revealed a significant correlation between multiplex and single stains (p < 0.01) as well as a concordant staining pattern for ERG (n = 14 prostate cancers were identified ERG positive with both methods). Our proof of concept study demonstrates a robust staining pattern of all five different antibodies with this newly developed 5x MPX IHC. This approach facilitates the recognition of prostate cancer, in particular by adding PSMA in cases with low p504s expression.
CitationRüschoff JH, Stratton S, Roberts E, Clark S, Sebastiao N, Fankhauser CD, et al. A novel 5x multiplex immunohistochemical staining reveals PSMA as a helpful marker in prostate cancer with low p504s expression. [Internet]. Vol. 228, Pathology - Research and Practice. Elsevier BV; 2021. p. 153667.
JournalPathology Research and Practice
- Basal cell cocktail (34betaE12 + p63) improves the detection of prostate basal cells.
- Authors: Zhou M, Shah R, Shen R, Rubin MA
- Issue date: 2003 Mar
- Use of p63/P504S monoclonal antibody cocktail in immunohistochemical staining of prostate tissue.
- Authors: Tacha DE, Miller RT
- Issue date: 2004 Mar
- Diagnostic utility of P504S/p63 cocktail, prostate-specific antigen, and prostatic acid phosphatase in verifying prostatic carcinoma involvement in seminal vesicles: a study of 57 cases of radical prostatectomy specimens of pathologic stage pT3b.
- Authors: Harvey AM, Grice B, Hamilton C, Truong LD, Ro JY, Ayala AG, Zhai QJ
- Issue date: 2010 Jul
- Prospective evaluation of AMACR (P504S) and basal cell markers in the assessment of routine prostate needle biopsy specimens.
- Authors: Browne TJ, Hirsch MS, Brodsky G, Welch WR, Loda MF, Rubin MA
- Issue date: 2004 Dec
- Performance comparison of anti-p504s (SP116) Rabbit Monoclonal Primary Antibody vs. Monoclonal Rabbit Anti-Human AMACR clone 13H4 when duplexed with VENTANA Basal Cell Cocktail (34βE12+p63) as a diagnostic aid for prostatic adenocarcinoma using immunohistochemistry.
- Authors: Lindner V, Waydelich A, Chen CC, Jones C, Stratton SP
- Issue date: 2021 Aug