A novel 5x multiplex immunohistochemical staining reveals PSMA as a helpful marker in prostate cancer with low p504s expression
Authors
Rüschoff, J. H.Stratton, S.
Roberts, E.
Clark, S.
Sebastiao, N.
Fankhauser, Christian D
Eberli, D.
Moch, H.
Wild, P. J.
Rupp, N. J.
Affiliation
Department of Pathology and Molecular Pathology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.Issue Date
2021
Metadata
Show full item recordAbstract
The ability to combine multiple immunohistochemical (IHC) markers within a single tissue section facilitates the evaluation and detection of co-expressions, while saving tissue. A newly developed 5x multiplex (MPX) IHC staining of five different IHC markers (Basal cell cocktail (34βE12 + p63), p504s (SP116), ERG (EPR3864), Ki-67 (30-9), PSMA (EP192)) was applied on whole sections of n = 37 radical prostatectomies (RPE) including normal and cancerous tissue. Four different colors including brown, magenta, yellow and teal coded for different stainings, whereas magenta was used twice for nuclear Ki-67 and cytosolic / membranous PSMA. The staining of multiplex IHC was compared to single stains of ERG, PSMA and p504s. The proper staining of the basal cell cocktail and Ki-67 could be assessed by internal positive controls in the multiplex staining. The proportion of PSMA and p504s expression revealed a significant correlation between multiplex and single stains (p < 0.01) as well as a concordant staining pattern for ERG (n = 14 prostate cancers were identified ERG positive with both methods). Our proof of concept study demonstrates a robust staining pattern of all five different antibodies with this newly developed 5x MPX IHC. This approach facilitates the recognition of prostate cancer, in particular by adding PSMA in cases with low p504s expression.Citation
Rüschoff JH, Stratton S, Roberts E, Clark S, Sebastiao N, Fankhauser CD, et al. A novel 5x multiplex immunohistochemical staining reveals PSMA as a helpful marker in prostate cancer with low p504s expression. [Internet]. Vol. 228, Pathology - Research and Practice. Elsevier BV; 2021. p. 153667.Journal
Pathology Research and PracticeDOI
10.1016/j.prp.2021.153667PubMed ID
34717149Additional Links
https://dx.doi.org/10.1016/j.prp.2021.153667Type
ArticleLanguage
enae974a485f413a2113503eed53cd6c53
10.1016/j.prp.2021.153667