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    A novel 5x multiplex immunohistochemical staining reveals PSMA as a helpful marker in prostate cancer with low p504s expression

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    Authors
    Rüschoff, J. H.
    Stratton, S.
    Roberts, E.
    Clark, S.
    Sebastiao, N.
    Fankhauser, Christian D
    Eberli, D.
    Moch, H.
    Wild, P. J.
    Rupp, N. J.
    Affiliation
    Department of Pathology and Molecular Pathology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
    Issue Date
    2021
    
    Metadata
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    Abstract
    The ability to combine multiple immunohistochemical (IHC) markers within a single tissue section facilitates the evaluation and detection of co-expressions, while saving tissue. A newly developed 5x multiplex (MPX) IHC staining of five different IHC markers (Basal cell cocktail (34βE12 + p63), p504s (SP116), ERG (EPR3864), Ki-67 (30-9), PSMA (EP192)) was applied on whole sections of n = 37 radical prostatectomies (RPE) including normal and cancerous tissue. Four different colors including brown, magenta, yellow and teal coded for different stainings, whereas magenta was used twice for nuclear Ki-67 and cytosolic / membranous PSMA. The staining of multiplex IHC was compared to single stains of ERG, PSMA and p504s. The proper staining of the basal cell cocktail and Ki-67 could be assessed by internal positive controls in the multiplex staining. The proportion of PSMA and p504s expression revealed a significant correlation between multiplex and single stains (p < 0.01) as well as a concordant staining pattern for ERG (n = 14 prostate cancers were identified ERG positive with both methods). Our proof of concept study demonstrates a robust staining pattern of all five different antibodies with this newly developed 5x MPX IHC. This approach facilitates the recognition of prostate cancer, in particular by adding PSMA in cases with low p504s expression.
    Citation
    Rüschoff JH, Stratton S, Roberts E, Clark S, Sebastiao N, Fankhauser CD, et al. A novel 5x multiplex immunohistochemical staining reveals PSMA as a helpful marker in prostate cancer with low p504s expression. [Internet]. Vol. 228, Pathology - Research and Practice. Elsevier BV; 2021. p. 153667.
    Journal
    Pathology Research and Practice
    URI
    http://hdl.handle.net/10541/624763
    DOI
    10.1016/j.prp.2021.153667
    PubMed ID
    34717149
    Additional Links
    https://dx.doi.org/10.1016/j.prp.2021.153667
    Type
    Article
    Language
    en
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.prp.2021.153667
    Scopus Count
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