• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    CHAMPion and WiSDen studies: investigating the relationship between a stromal wound healing phenotype and breast density

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Castle, John
    Blower, Emma
    Ogunlayi, H
    Kirwan, Cliona C
    Affiliation
    Manchester Cancer Research Centre
    Issue Date
    2021
    
    Metadata
    Show full item record
    Abstract
    Background: Tumour stroma resembles a �wound that will not heal�, with myo?broblasts phenotypically similar to cancer-associated ?broblasts (CAFs). Fibroblasts produce collagen and are hyperactivated in high breast density tissue. Breast cancer ?broblasts show increasing expression of procoagulant and CAF markers in the normal-DCIS (ductal carcinoma in situ, breast cancer precursor)-invasive cancer transition. High mammographic density is associated with an increased risk of breast cancer, but the mechanism for this is unclear.Purpose: We hypothesise that high density breast tissue has a wound-like, procoagulant stroma, causing increased collagen formation (visible mammographic density) and promoting breast cancer development, including the transition from DCIS to invasion. This may provide novel therapeutic options for breast cancer prevention. Methods: Fresh tumour/DCIS tissue plus distant normal tissue are collected from patients undergoing mastectomies at Wythenshawe Hospital with the support of pathologists and the Manchester Cancer Research Centre Biobank. Primary broblasts are grown from these and characterised with their e?ect on established breast cancer cell lines investigated. In the ?nal phase of the study, mammographic density and residual FFPE tissue will be compared to the experimental results. Results: Primary ?broblast culture is achievable for most tissue samples, with a bank of frozen cell stocks, conditioned media, lysates and FFPE built up for later study phases. Initial functional experiments using the modi?ed prothrombin time assay indicate ?broblasts cells and their secreted factors in conditioned media initiate coagulation. Conclusions: Cultured primary ?broblasts have been shown to be functionally procoagulant, as suggested by earlier work on ?xed tissue. Functional experiments with the cultured ?broblasts and comparisons to mammographic density will be carried out in the next phases of this study.
    Citation
    Castle J, Blower E, Ogunlayi H, Kirwan CC. CHAMPion and WiSDen Studies: Investigating the Relationship Between a Stromal Wound Healing Phenotype and Breast Density. Journal of Pathology. 2021;255:S4-S.
    Journal
    Journal of Pathology
    URI
    http://hdl.handle.net/10541/624746
    Type
    Other
    Language
    en
    Collections
    All Paterson Institute for Cancer Research

    entitlement

     
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.