Enhancer recruitment of transcription repressors RUNX1 and TLE3 by mis-expressed FOXC1 blocks differentiation in acute myeloid leukemia
Authors
Simeoni, FabrizioRomero-Camarero, Isabel
Camera, Francesco
Amaral, Fabio
Sinclair, Oliver J
Papachristou, E. K.
Spencer, Gary J
Lie-A-Ling, Michael
Lacaud, Georges
Wiseman, Daniel H
Carroll, J. S.
Somervaille, Tim C P
Affiliation
Leukaemia Biology Laboratory, Cancer Research UK Manchester Institute, The University of Manchester, Manchester M20 4GJIssue Date
2021
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Despite absent expression in normal hematopoiesis, the Forkhead factor FOXC1, a critical mesenchymal differentiation regulator, is highly expressed in ?30% of HOXAhigh acute myeloid leukemia (AML) cases to confer blocked monocyte/macrophage differentiation. Through integrated proteomics and bioinformatics, we find that FOXC1 and RUNX1 interact through Forkhead and Runt domains, respectively, and co-occupy primed and active enhancers distributed close to differentiation genes. FOXC1 stabilizes association of RUNX1, HDAC1, and Groucho repressor TLE3 to limit enhancer activity: FOXC1 knockdown induces loss of repressor proteins, gain of CEBPA binding, enhancer acetylation, and upregulation of nearby genes, including KLF2. Furthermore, it triggers genome-wide redistribution of RUNX1, TLE3, and HDAC1 from enhancers to promoters, leading to repression of self-renewal genes, including MYC and MYB. Our studies highlight RUNX1 and CEBPA transcription factor swapping as a feature of leukemia cell differentiation and reveal that FOXC1 prevents this by stabilizing enhancer binding of a RUNX1/HDAC1/TLE3 transcription repressor complex to oncogenic effect.Citation
Simeoni F, Romero-Camarero I, Camera F, Amaral FMR, Sinclair OJ, Papachristou EK, et al. Enhancer recruitment of transcription repressors RUNX1 and TLE3 by mis-expressed FOXC1 blocks differentiation in acute myeloid leukemia. Vol. 36, Cell Reports. Elsevier BV; 2021. p. 109725.Journal
Cell ReportsDOI
10.1016/j.celrep.2021.109725PubMed ID
34551306Additional Links
https://dx.doi.org/10.1016/j.celrep.2021.109725Type
ArticleLanguage
enae974a485f413a2113503eed53cd6c53
10.1016/j.celrep.2021.109725
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