Evaluation of senescent cells in intervertebral discs by lipofuscin staining
Gorgoulis, Vassilis G
AffiliationMolecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, Athens, Greece
MetadataShow full item record
AbstractIntervertebral disc (IVD) degeneration is considered an important contributor of low back pain, a major age-related disease. Interestingly, an unprecedented high number of senescent cells has been reported in aged and degenerated IVDs, most probably affecting tissue homeostasis. In previous studies classical markers of cellular senescence have been used, such as SA-β-gal staining or p16Ink4a expression. Aim of the presented study was a re-evaluation of the number of senescent IVD cells by using a newly established staining procedure for lipofuscin, based on a Sudan Black-B analogue (GL13), which can be used in fresh, as well as in fixed and embedded tissues. In cultures of senescent rat and human IVD cells both SA-β-gal and GL13 gave similar percentages of senescent cells. Similarly, in fresh tissues from old rats the ratios of senescent cells were high with both detection procedures. Finally, in formalin-fixed and paraffin-embedded tissues from humans, a significant increased number of GL13-positive cells was found in herniated tissues, as compared to apparently normal ones, while similar numbers of p16Ink4a-positive cells were observed. These data confirm the significantly enhanced number of senescent cells in aged and degenerated IVDs, most probably contributing to the degeneration of this tissue.
CitationVeroutis D, Kouroumalis A, Lagopati N, Polyzou A, Chamilos C, Papadodima S, et al. Evaluation of senescent cells in intervertebral discs by lipofuscin staining. Mechanisms of Ageing and Development. 2021 Oct;199:111564.
JournalMechanisms of Ageing and Development
- Toll-like receptor 2 induced senescence in intervertebral disc cells of patients with back pain can be attenuated by o-vanillin.
- Authors: Mannarino M, Cherif H, Li L, Sheng K, Rabau O, Jarzem P, Weber MH, Ouellet JA, Haglund L
- Issue date: 2021 Apr 16
- p16<sup>Ink4a</sup> deletion in cells of the intervertebral disc affects their matrix homeostasis and senescence associated secretory phenotype without altering onset of senescence.
- Authors: Novais EJ, Diekman BO, Shapiro IM, Risbud MV
- Issue date: 2019 Sep
- Senescence in cells of the aging and degenerating intervertebral disc: immunolocalization of senescence-associated beta-galactosidase in human and sand rat discs.
- Authors: Gruber HE, Ingram JA, Norton HJ, Hanley EN Jr
- Issue date: 2007 Feb 1
- Senescence mechanisms of nucleus pulposus chondrocytes in human intervertebral discs.
- Authors: Kim KW, Chung HN, Ha KY, Lee JS, Kim YY
- Issue date: 2009 Aug
- Specific lipofuscin staining as a novel biomarker to detect replicative and stress-induced senescence. A method applicable in cryo-preserved and archival tissues.
- Authors: Georgakopoulou EA, Tsimaratou K, Evangelou K, Fernandez Marcos PJ, Zoumpourlis V, Trougakos IP, Kletsas D, Bartek J, Serrano M, Gorgoulis VG
- Issue date: 2013 Jan