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dc.contributor.authorBelow, Christopher R
dc.contributor.authorKelly, Joanna
dc.contributor.authorBrown, A.
dc.contributor.authorHumphries, J. D.
dc.contributor.authorHutton, Colin
dc.contributor.authorXu, Jingshu
dc.contributor.authorLee, Brian Y
dc.contributor.authorCintas, Celia
dc.contributor.authorZhang, Xiaohong
dc.contributor.authorHernandez-Gordillo, V.
dc.contributor.authorStockdale, L.
dc.contributor.authorGoldsworthy, M. A.
dc.contributor.authorGeraghty, J.
dc.contributor.authorFoster, L.
dc.contributor.authorO'Reilly, D. A.
dc.contributor.authorSchedding, B.
dc.contributor.authorAskari, J.
dc.contributor.authorBurns, J.
dc.contributor.authorHodson, N.
dc.contributor.authorSmith, Duncan L
dc.contributor.authorLally, Catherine
dc.contributor.authorAshton, Garry
dc.contributor.authorKnight, D.
dc.contributor.authorMironov, A.
dc.contributor.authorBanyard, Antonia
dc.contributor.authorEble, J. A.
dc.contributor.authorMorton, J. P.
dc.contributor.authorHumphries, M. J.
dc.contributor.authorGriffith, L. G.
dc.contributor.authorJørgensen, Claus
dc.date.accessioned2021-09-30T11:56:12Z
dc.date.available2021-09-30T11:56:12Z
dc.date.issued2021en
dc.identifier.citationBelow CR, Kelly J, Brown A, Humphries JD, Hutton C, Xu J, et al. A microenvironment-inspired synthetic three-dimensional model for pancreatic ductal adenocarcinoma organoids. Nat Mater. 2021 Sep 13.en
dc.identifier.pmid34518665en
dc.identifier.doi10.1038/s41563-021-01085-1en
dc.identifier.urihttp://hdl.handle.net/10541/624640
dc.description.abstractExperimental in vitro models that capture pathophysiological characteristics of human tumours are essential for basic and translational cancer biology. Here, we describe a fully synthetic hydrogel extracellular matrix designed to elicit key phenotypic traits of the pancreatic environment in culture. To enable the growth of normal and cancerous pancreatic organoids from genetically engineered murine models and human patients, essential adhesive cues were empirically defined and replicated in the hydrogel scaffold, revealing a functional role of laminin-integrin α3/α6 signalling in establishment and survival of pancreatic organoids. Altered tissue stiffness-a hallmark of pancreatic cancer-was recapitulated in culture by adjusting the hydrogel properties to engage mechano-sensing pathways and alter organoid growth. Pancreatic stromal cells were readily incorporated into the hydrogels and replicated phenotypic traits characteristic of the tumour environment in vivo. This model therefore recapitulates a pathologically remodelled tumour microenvironment for studies of normal and pancreatic cancer cells in vitro.en
dc.language.isoenen
dc.relation.urlhttps://dx.doi.org/10.1038/s41563-021-01085-1en
dc.titleA microenvironment-inspired synthetic three-dimensional model for pancreatic ductal adenocarcinoma organoidsen
dc.typeArticleen
dc.contributor.departmentCancer Research UK Manchester Institute, The University of Manchester, Manchester, UKen
dc.identifier.journalNature Materialsen
dc.description.noteen]


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