Understanding small cell lung cancer metastasis using circulating tumor cell (CTC)-derived tumor explant (CDX) models
Singh, S. K.
Frese, Kristopher K
Blackhall, Fiona H
Simpson, Kathryn L
AffiliationCRUK Manchester Institute Cancer Biomarker Centre, Manchester,
MetadataShow full item record
AbstractSmall cell lung cancer (SCLC) accounts for about 13% of all malignant lung tumors and is characterized by an extremely low survival rate and widespread early dissemination. Over two-thirds of SCLC patients are diagnosed with metastases in multiple organs; typically liver, lymph nodes and brain. The number and site of secondary lesions at diagnosis correlates with unfavorable disease outcome. Studying metastasis in SCLC is extremely challenging, as metastatic tumor samples are rarely obtained from these patients. Our laboratory has developed a patient-faithful biobank of >45 SCLC circulating tumor cell (CTC)-derived explants (CDX) models with which to study SCLC1. We implemented a resection protocol using these models to study the metastatic process in SCLC by interrogating the genetic and phenotypic components of metastatic cells using combinations of tissue pathology and next generation sequencing methods. Upon resection of the subcutaneous tumor, we detect the presence of overt macrometastatic lesions in multiple models, which in most cases retain matched organ tropism to that observed in the patient. Whereas all models so far tested disseminated to the lungs, the ATOH1 subtye2 models CDX17, CDX17P, CDX25 and CDX30P predominantly grew in the liver, and CDX3 and CDX3P (ASCL1 subtype3) preferentially colonized the brain despite having a much slower growth. CDX SCLC cells can be isolated from subcutaneous tumors and orthotopically implanted, recapitulating the same tropism. Finally, CDX cells labelled ex vivo can be re-implanted directly into the bloodstream or into the brain parenchyma giving rise to SCLC secondary tumors, providing us with an excellent tool to monitor and study several steps of the metastatic cascade, including extravasation and brain colonization. We report for the first time, brain tropic mouse models of SCLC. Molecular studies are underway to investigate the underlying genetic determinants and mechanisms underpinning metastatic spread of SCLC via transcriptomic studies of matched subcutaneous and metastatic tissues and CTCs in CDX. In parallel, CDX3P is being used to identify markers of pre-metastatic disease and brain colonization at the single cell level to identify novel therapeutic strategies and biomarkers that may benefit the significant number of SCLC patients who present with brain lesions.
CitationPeiris-Pagès M, Revill M, Morgan D, Brown S, Galvin M, Priest L, et al. Abstract 2874: Understanding small cell lung cancer metastasis using circulating tumor cell (CTC)-derived tumor explant (CDX) models. In: Tumor Biology. American Association for Cancer Research; 2021.