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    Systematic roadmap for cancer drug screening using zebrafish embryo xenograft cancer models: melanoma cell line as a case study

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    Authors
    Letrado, P.
    Mole, H.
    Montoya, M.
    Palacios, I.
    Barriuso, Jorge
    Hurlstone, A.
    Díez-Martínez, R
    Oyarzabal, J.
    Affiliation
    Ikan Biotech SL, Centro Europeo de Empresas e Innovación de Navarra (CEIN), 31110 Noain, Spain
    Issue Date
    2021
    
    Metadata
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    Abstract
    Zebrafish embryo tumor transplant models are widely utilized in cancer research. Compared with traditional murine models, the small size and transparency of zebrafish embryos combined with large clutch sizes that increase statistical power and cheap husbandry make them a cost-effective and versatile tool for in vivo drug discovery. However, the lack of a comprehensive analysis of key factors impacting the successful use of these models impedes the establishment of basic guidelines for systematic screening campaigns. Thus, we explored the following crucial factors: (i) user-independent inclusion criteria, focusing on sample homogeneity; (ii) metric definition for data analysis; (iii) tumor engraftment criteria; (iv) image analysis versus quantification of human cancer cells using qPCR (RNA and gDNA); (v) tumor implantation sites; (vi) compound distribution (intratumoral administration versus alternative inoculation sites); and (vii) efficacy (intratumoral microinjection versus compound solution in media). Based on these analyses and corresponding assessments, we propose the first roadmap for systematic drug discovery screening in zebrafish xenograft cancer models using a melanoma cell line as a case study. This study aims to help the wider cancer research community to consider the adoption of this versatile model for cancer drug screening projects.
    Citation
    Letrado P, Mole H, Montoya M, Palacios I, Barriuso J, Hurlstone A, et al. Systematic Roadmap for Cancer Drug Screening Using Zebrafish Embryo Xenograft Cancer Models: Melanoma Cell Line as a Case Study. Cancers. 2021 Jul 23;13(15):3705.
    Journal
    Cancers
    URI
    http://hdl.handle.net/10541/624522
    DOI
    10.3390/cancers13153705
    PubMed ID
    34359605
    Additional Links
    https://dx.doi.org/10.3390/cancers13153705
    Type
    Article
    Language
    en
    ae974a485f413a2113503eed53cd6c53
    10.3390/cancers13153705
    Scopus Count
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