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dc.contributor.authorAnjanappa, Milan
dc.contributor.authorRoberts, D.
dc.contributor.authorReeves, K.
dc.contributor.authorSong, Yee Pei
dc.contributor.authorAkturk, N
dc.contributor.authorChoudhury, Ananya
dc.date.accessioned2021-07-28T12:41:39Z
dc.date.available2021-07-28T12:41:39Z
dc.date.issued2020en
dc.identifier.citationParry M, Cowling T, Sujenthiran A, Nossiter J, Berry B, Cathcart P, et al. PO-1178: Identifying skeletal-related events for prostate cancer in routinely collected hospital data. Radiotherapy and Oncology . 2020 Nov;152:S620.en
dc.identifier.urihttp://hdl.handle.net/10541/624160
dc.description.abstractPurpose or Objective The presence of tumour infiltrating lymphocytes (TILs) is prognostic in a number of solid tumours such as colon, lung and breast. An immune supressed tumour microenvironment is a poor prognostic factor thought to be due to a limited host response to cancer cell death. The immune response gene analysis enables characterisation of the TILs and the responsible pathways. Bladder cancer is known to be influenced by immune modulation. We hypothesised that the immune genotype of muscleinvasive bladder cancers treated with radical chemoradiotherapy would stratify for distant failure. Material and Methods Between 2012 and 2014, fifty patients with muscle invasive bladder cancer underwent radical radiotherapy (52.5Gy in 20 fractions) with concurrent gemcitabine (100mg/m2) as per institutional protocol (NCT01343121). Forty-eight tumour tissue sample blocks were available for analysis. Next generation sequencing of transcriptomic RNA was performed evaluating the expression of 395 immune response genes. Gene expression was categorised relative to validated house-keeping genes and analysed with respect to patients who had relapsed with distant metastases. Results After 3 years of follow up, 6 patients had developed distant metastases. In these patients, CD3 and IL10 expression was reduced indicating a low T-cell and monocyte infiltrate. MTOR, PIK3CA and PTPN11 were elevated suggesting activation of signalling pathways often found in aggressive cancers. Conclusion This study is hypothesis generating, but provides data supporting a relationship between the immune tumour microenvironment and poor response to treatment and development of distant metastases. With the PIK3CAMTOR- AKT and RAS-MAPK pathways implicated with an increased aggressive phenotype and resistance to anti- PD1/PDL1 therapy, immune response gene signatures should be investigated as both prognostic and predictive biomarkers in bladder cancer.en
dc.language.isoenen
dc.titleImmune response gene expression analysis and response to radical chemoradiation in bladder canceren
dc.typeMeetings and Proceedingsen
dc.contributor.departmentThe Christie NHS Foundation Trust, Clinical Oncology, Manchester,en
dc.identifier.journalRadiotherapy and Oncologyen
dc.description.noteen]


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